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GAD65 Polyclonal Antibody

GAD65 Antibody in Immunocytochemistry (ICC/IF)

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GAD65 Antibody (20746-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 20746-1-AP (GAD65 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

GAD65 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20746-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to GAD2.

Target Information

GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Mon Aug 01 00:00:00 UTC 2022

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Nature cell biology

Huang D,Wang Y,Thompson JW,Yin T,Alexander PB,Qin D,Mudgal P,Wu H,Liang Y,Tan L,Pan C,Yuan L,Wan Y,Li QJ,Wang XF

Tue Feb 01 00:00:00 UTC 2022

Intrathecally administered pizotifen alleviates neuropathic and inflammatory pain in mice by enhancing GABAergic inhibition.

Neuroscience letters

Li J,Zhang X,Xu C,Liu YX,Ge C,Zhao Z,Zhu YB,Bao HG

Fri Apr 01 00:00:00 UTC 2022

BNST_AV ^GABA-PVN^CRF Circuit Regulates Visceral Hypersensitivity Induced by Maternal Separation in Vgat-Cre Mice.

Frontiers in pharmacology

Huang ST,Song ZJ,Liu Y,Luo WC,Yin Q,Zhang YM

Tue Apr 06 00:00:00 UTC 2021

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Aging

Wu X,Sheng H,Wang L,Xia P,Wang Y,Yu L,Lv W,Hu J

Fri Mar 26 00:00:00 UTC 2021

Spinal GABAergic neurons are under feed-forward inhibitory control driven by Aδ and C fibers in Gad2 td-Tomato mice.

Molecular pain

Liu P,Zhang X,He X,Jiang Z,Wang Q,Lu Y

Thu Oct 14 00:00:00 UTC 2021

Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.

Frontiers in pharmacology

Zhu X,Li T,Hu E,Duan L,Zhang C,Wang Y,Tang T,Yang Z,Fan R

Fri Feb 04 00:00:00 UTC 2022

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Aging

Wang D,Zhang H,Zeng M,Tang X,Zhu X,Guo Y,Qi L,Xie Y,Zhang M,Chen D

Fri Mar 26 00:00:00 UTC 2021

A sex-dependent delayed maturation of visual plasticity induced by adverse experiences in early childhood.

Neurobiology of stress

Liu Y,Wang Z,Zhang X,Li S,Wu W,Li X,Yang Y

Sun Nov 01 00:00:00 UTC 2020

Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system.

Clinical science (London, England : 1979)

Chen K,Sun D,Qu S,Chen Y,Wang J,Zhou L,Jose PA,Yang Y,Zeng C

Fri May 31 00:00:00 UTC 2019

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Scientific reports

Chen HH,Cheng PW,Ho WY,Lu PJ,Lai CC,Tseng YM,Fang HC,Sun GC,Hsiao M,Liu CP,Tseng CJ

Mon Dec 05 00:00:00 UTC 2016

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Scientific reports

Chen HH,Cheng PW,Ho WY,Lu PJ,Lai CC,Tseng YM,Fang HC,Sun GC,Hsiao M,Liu CP,Tseng CJ

Mon Dec 05 00:00:00 UTC 2016

The distinct role of NR2B subunit in the enhancement of visual plasticity in adulthood.

Molecular brain

Liu H,Li Y,Wang Y,Wang X,An X,Wang S,Chen L,Liu G,Yang Y

Wed Aug 19 00:00:00 UTC 2015

The distinct role of NR2B subunit in the enhancement of visual plasticity in adulthood.

Molecular brain

Liu H,Li Y,Wang Y,Wang X,An X,Wang S,Chen L,Liu G,Yang Y

Wed Aug 19 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2

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Gene Aliases: 6330404F12Rik; GAD(65); Gad-2; GAD2; GAD65

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UniProt ID: (Human) Q05329, (Mouse) P48320, (Rat) Q05683

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Entrez Gene ID: (Human) 2572, (Mouse) 14417, (Rat) 24380

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Function(s)

glutamate decarboxylase activity protein binding glutamate binding pyridoxal phosphate binding protein heterodimerization activity catalytic activity lyase activity carboxy-lyase activity

Process(es)

glutamate decarboxylation to succinate carboxylic acid metabolic process neurotransmitter biosynthetic process chemical synaptic transmission response to drug neurotransmitter secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GAD65 Polyclonal Antibody

GAD65 Antibody (20746-1-AP) in ICC/IF

Product Details

20746-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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