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Invitrogen
GAD65/GAD67 Recombinant Rabbit Monoclonal Antibody (HL1181)
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FIGURE: 1 / 9
GAD65/GAD67 Antibody (MA5-46950) in ICC/IF
Immunofluorescent analysis of GAD65/GAD67 in DIV9 rat E18 primary cortical neuron cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD65 + GAD67 stained by GAD65 + GAD67 monoclonal antibody (Product # MA5-46950) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Product Details
MA5-46950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1181
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2938022
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa glutamic acid decarboxylase; 67 kDa glutamic acid decarboxylase; EC 4.1.1.15; FLJ45882; GAD 1; GAD 2; GAD 65; GAD 67; GAD-65; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 1; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65
Gene Aliases: 6330404F12Rik; CPSQ1; EP10; GAD; GAD(65); Gad-1; Gad-2; GAD1; GAD2; GAD25; GAD44; GAD65; GAD67; SCP; zgc:112198
UniProt ID: (Human) Q99259, (Human) Q05329, (Mouse) P48318, (Rat) P18088, (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Human) 2571, (Human) 2572, (Mouse) 14415, (Rat) 24379, (Mouse) 14417, (Rat) 24380, (Zebrafish) 550403
glutamate catabolic process
glutamate decarboxylation to succinate
chemical synaptic transmission
neurotransmitter secretion
gamma-aminobutyric acid biosynthetic process
protein-pyridoxal-5-phosphate linkage
neurotransmitter biosynthetic process
response to drug
carboxylic acid metabolic process
social behavior
locomotory exploration behavior
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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