Invitrogen

GADD34 Polyclonal Antibody

5 Published Figures

8 References

View all (23) GADD34 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (5)

GADD34 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

GADD34 Antibody (PA1-139) in ICC/IF

Immunofluorescent analysis of GADD34 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a GADD34 polyclonal antibody (Product # PA1-139) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:500 for 30 minutes at room t... View More

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Product Details

PA1-139

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:50-1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to amino acids C R(595) I T Q A Q E E L S P C L T P A A R(612) from human GADD34

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2539894

Product Specific Information

Western blot analysis of PA1-139 detects an ~73 kDa protein in HeLa cells. PA1-139 shows specificity to GADD34 and can be blocked by incubating with an abundance of the immunizing peptide.

Target Information

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

4-phenylbutyric acid-Identity crisis; can it act as a translation inhibitor?

Aging cell

Stein D,Slobodnik Z,Tam B,Einav M,Akabayov B,Berstein S,Toiber D

PA1-139 was used in Western Blot to show that 4PBA is a conserved weak inhibitor of mRNA translation, both in vitro and in cellular systems, and furthermore-it does not promote protein folding nor prevents aggregation.

Thu Dec 01 00:00:00 UTC 2022

Inhibition of eIF2α Phosphorylation by Peste des Petits Ruminant Virus Phosphoprotein Facilitates Viral Replication.

Frontiers in veterinary science

Alfred N,Qian B,Qin X,Yin X,Prajapati M,Dou Y,Li Y,Zhang Z

Published figure using GADD34 polyclonal antibody (Product # PA1-139) in Western Blot

Sat Jul 24 00:00:00 UTC 2021

Inhibition of eIF2α Phosphorylation by Peste des Petits Ruminant Virus Phosphoprotein Facilitates Viral Replication.

Frontiers in veterinary science

Alfred N,Qian B,Qin X,Yin X,Prajapati M,Dou Y,Li Y,Zhang Z

Published figure using GADD34 polyclonal antibody (Product # PA1-139) in Western Blot

Sat Jul 24 00:00:00 UTC 2021

Inhibition of eIF2α Phosphorylation by Peste des Petits Ruminant Virus Phosphoprotein Facilitates Viral Replication.

Frontiers in veterinary science

Alfred N,Qian B,Qin X,Yin X,Prajapati M,Dou Y,Li Y,Zhang Z

Published figure using GADD34 polyclonal antibody (Product # PA1-139) in Western Blot

Sat Jul 24 00:00:00 UTC 2021

Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells.

Cell death & disease

Attwood KM,Robichaud A,Westhaver LP,Castle EL,Brandman DM,Balgi AD,Roberge M,Colp P,Croul S,Kim I,McCormick C,Corcoran JA,Weeks A

PA1-139 was used in Western Blot to indicate that modulating the stress response can be used to exploit the hypoxic niche of GBM tumors, causing cell death by disrupting pro-survival stress responses and control of protein synthesis.

Tue Nov 17 00:00:00 UTC 2020

Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells.

Cell death & disease

Attwood KM,Robichaud A,Westhaver LP,Castle EL,Brandman DM,Balgi AD,Roberge M,Colp P,Croul S,Kim I,McCormick C,Corcoran JA,Weeks A

Tue Nov 17 00:00:00 UTC 2020

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA1-139 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 UTC 2020

GADD34 is a modulator of autophagy during starvation.

Science advances

Gambardella G,Staiano L,Moretti MN,De Cegli R,Fagnocchi L,Di Tullio G,Polletti S,Braccia C,Armirotti A,Zippo A,Ballabio A,De Matteis MA,di Bernardo D

PA1-139 was used in Immunocytochemistry-immunoflourescence to show TFEB directly activates expression of GADD34 to suppress protein synthesis inhibition.

Tue Sep 01 00:00:00 UTC 2020

EIF1AX and RAS Mutations Cooperate to Drive Thyroid Tumorigenesis through ATF4 and c-MYC.

Cancer discovery

Krishnamoorthy GP,Davidson NR,Leach SD,Zhao Z,Lowe SW,Lee G,Landa I,Nagarajah J,Saqcena M,Singh K,Wendel HG,Dogan S,Tamarapu PP,Blenis J,Ghossein RA,Knauf JA,Rätsch G,Fagin JA

PA1-139 was used in Western Blotting to describe the identification of the key signaling drivers of transformation by EIF1AX mutants, particularly EIF1AX-A113splice, alone and in the context of RAS, and the therapeutic dependencies they confer.

Fri Feb 01 00:00:00 UTC 2019

Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome.

American journal of human genetics

Poli MC,Ebstein F,Nicholas SK,de Guzman MM,Forbes LR,Chinn IK,Mace EM,Vogel TP,Carisey AF,Benavides F,Coban-Akdemir ZH,Gibbs RA,Jhangiani SN,Muzny DM,Carvalho CMB,Schady DA,Jain M,Rosenfeld JA,Emrick L,Lewis RA,Lee B,Zieba BA,Küry S,Krüger E,Lupski JR,Bostwick BL,Orange JS

PA1-139 was used in Western Blotting to characterise the Proteasome maturation protein-related autoinflammation and immune dysregulation disease discovered in two unrelated individuals with a unique constellation of early-onset combined immunodeficiency, inflammatory neutrophilic dermatosis, and autoimmunity.

Thu Jun 07 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response gene 116; Myeloid differentiation primary response protein MyD116; Myeloid differentiation primary response protein MyD116 homolog; PR15A; Protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A; protein phosphatase 1, regulatory subunit 15A

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Gene Aliases: 9630030H21; GADD34; Myd116; PPP1R15A

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UniProt ID: (Human) O75807, (Mouse) P17564

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Entrez Gene ID: (Human) 23645, (Mouse) 17872

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Function(s)

protein binding protein phosphatase 1 binding protein phosphatase type 1 regulator activity protein kinase binding protein phosphatase type 1 activator activity protein phosphatase regulator activity protein phosphatase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GADD34 Polyclonal Antibody

GADD34 Antibody (PA1-139) in ICC/IF

Product Details

PA1-139

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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