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Invitrogen
GADD45A Polyclonal Antibody
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Product Details
PA5-88248
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_0019151)
Conjugate
Form
Liquid
Concentration
1.44 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804773
Product Specific Information
Immunogen sequence: MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER; Positive Samples: HeLa, MCF7; Cellular Location: Nucleus
Target Information
GADD45 alpha, beta and gamma are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents. GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase. GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DDIT 1; DDIT-1; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; DNA-damage inducible transcript 1; DNA-damage-inducible transcript 1; Gadd45alpha; Growth arrest and DNA damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible, alpha
Gene Aliases: AA545191; DDIT1; GADD45; GADD45A
UniProt ID: (Human) P24522, (Mouse) P48316
Entrez Gene ID: (Human) 1647, (Mouse) 13197, (Rat) 25112
activation of MAPKKK activity
negative regulation of protein kinase activity
response to stress
cellular response to DNA damage stimulus
cell cycle
cell cycle arrest
centrosome cycle
signal transduction in response to DNA damage
positive regulation of apoptotic process
positive regulation of JNK cascade
regulation of cell cycle
cellular response to ionizing radiation
mitotic cell cycle arrest
positive regulation of p38MAPK cascade
positive regulation of reactive oxygen species metabolic process
G2/M transition of mitotic cell cycle
cellular response to mechanical stimulus
regulation of cyclin-dependent protein serine/threonine kinase activity
DNA repair
apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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