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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GAPDH Antibodies

          Proteintech

          GAPDH Monoclonal Antibody (1E6D9)

          8,519 References
          View all (118) GAPDH antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite GAPDH Monoclonal Antibody (1E6D9)

          • Antibody Testing Data (21)
          GAPDH Antibody in Immunocytochemistry (ICC/IF)
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          GAPDH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 21

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          GAPDH Antibody (60004-1-IG) in ICC/IF

          Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-IG (GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IGG (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GAPDH Antibody in Immunocytochemistry (ICC/IF)
          GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Western Blot (WB)
          GAPDH Antibody in Flow Cytometry (Flow)
          GAPDH Antibody in Immunoprecipitation (IP)

          Product Details

          60004-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000-1:50,000
          View 7,920 publications 7,920 publications

          Immunohistochemistry (IHC)

          -
          View 7 publications 7 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 15 publications 15 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 36 publications 36 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 232 publications 232 publications

          Not applicable (N/A)

          -
          View 305 publications 305 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Plant, Rat, Yeast, Zebrafish

          Published species

          Avian, Bovine, Chicken, Deer, Dog, Fish, Fungi, Hamster, Human, Insect, Mammal, Mouse, Nematode, Pig, Rat, Sheep, Xenopus, Yeast, Zebrafish

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1E6D9

          Immunogen

          GAPDH Fusion Protein Ag0766 (1-335 aa encoded by BC004109)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite 594
          • CoraLite Plus 488
          • CoraLite Plus 647
          • CoraLite Plus 670
          • HRP
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

          Immunogen sequence: MGKVKVGVN GFGRIGRLVT RAAFNSGKVD IVAINDPFID LNYMVYMFQY DSTHGKFHGT VKAENGKLVI NGNPITIFQE RDPSKIKWGD AGAEYVVEST GVFTTMEKAG AHLQGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIISN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGALQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTAN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTEH QVVSSDFNSD THSSTFDAGA GIALNDHFVK LISWYDNEFG YSNRVVDLMA HMASKE (1-335 aa encoded by B C004109)

          Target Information

          GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

          View more View less

          Gene Aliases: BARS-38; bb02e05; cb350; cb609; CDABP0047; fb71f08; fk58c09; G3PD; g3pdh; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908

          View more View less

          UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467, (Zebrafish) Q5XJ10, (Zebrafish) Q5MJ86

          View more View less

          Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447, (Zebrafish) 317743, (Zebrafish) 406367

          View more View less

          Function(s)
          glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor nucleotide binding transferase activity
          Process(es)
          gluconeogenesis spermatid development sperm motility positive regulation of glycolytic process canonical glycolysis glucose metabolic process glycolytic process oxidation-reduction process microtubule cytoskeleton organization regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process cellular response to interferon-gamma carbohydrate metabolic process apoptotic process multicellular organism development response to ammonium ion regulation of translation
          It has to be done as per old AB suggested Products section.
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