Invitrogen

GAPDH Monoclonal Antibody (FF26A), eBioscience™

FIGURE: 1 / 7

GAPDH Antibody (14-9523-82) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), MDA-MB-231 (Lane 2) and COS-7 (Lane 3). The blot was probed with Anti-GAPDH Monoclonal Antibody (Product # 14-9523-80, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to GAPDH was observed across the cell lines tested.

Product Image Gallery

GAPDH Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with alpha GAPDH siRNA and loss of signal was observed in Western Blot using Anti-GAPDH Monoclonal Antibody (Product # 14-9523-82). {KD}

Product Details

14-9523-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 9 publications 9 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

FF26A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11151331

Product Specific Information

Description: This FF26A monoclonal antibody reacts with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key component of glycolysis. This 36 kDa enzyme is constitutively expressed in all tissues albeit at different levels. Measurement of GAPDH levels is often performed as a control for protein loading in experiments assessing differential protein expression.

The FF26A antibody is crossreactive to mouse GAPDH.

Applications Reported: This FF26A antibody has been reported for use in western blotting.

Applications Tested: This FF26A antibody has been tested by immunoblotting of cell lysates. This can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long non-coding RNA MEG3 promotes tumor necrosis factor-alpha induced oxidative stress and apoptosis in interstitial cells of cajal via targeting the microRNA-21 /I-kappa-B-kinase beta axis.

Bioengineered

Li J,Bai J,Tuerdi N,Liu K

14-9523-82 was used in Western Blotting to conclude that MEG3 mediates inflammation, oxidative stress and apoptosis in TNF-α-treated ICC via the miR-21/IKKB-NF-κB axis.

Fri Apr 01 00:00:00 UTC 2022

Melatonin Protects Against Ischemic Brain Injury by Modulating PI3K/AKT Signaling Pathway via Suppression of PTEN Activity.

ASN neuro

Ran Y,Ye L,Ding Z,Gao F,Yang S,Fang B,Liu Z,Xi J

Published figure using GAPDH monoclonal antibody (Product # 14-9523-82) in Western Blot

Thu Jan 13 00:00:00 UTC 2022

Antitumor activity of the dual BET and CBP/EP300 inhibitor NEO2734.

Blood advances

Spriano F,Gaudio E,Cascione L,Tarantelli C,Melle F,Motta G,Priebe V,Rinaldi A,Golino G,Mensah AA,Aresu L,Zucca E,Pileri S,Witcher M,Brown B,Wahlestedt C,Giles F,Stathis A,Bertoni F

14-9523 was used in Western Blotting to characterize the antitumor activity of the pan BET/CBP/EP300 inhibitor NEO2734 and provide insights into its mechanism of action through bromodomain-binding assays, in vitro and in vivo treatments of cancer cell lines, immunoblotting, and transcriptome analyses.

Tue Sep 08 00:00:00 UTC 2020

miR-16 enhances miR-302/367-induced reprogramming and tumor suppression in breast cancer cells.

IUBMB life

Hoseinbeyki M,Taha MF,Javeri A

14-9523-82 was used in Western Blot to study the impact of exogenous induction of miR-16 in MDA-MB-231 and SK-BR-3 breast cancer cells following overexpression of miR-302/367 cluster and investigate whether transfection of these cells by a mature miR-16 mimic could affect the reprogramming state of the cells and their tumorigenicity.

Fri May 01 00:00:00 UTC 2020

Rap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation.

Cell death & disease

Ding Y,Liang X,Zhang Y,Yi L,Shum HC,Chen Q,Chan BP,Fan H,Liu Z,Tergaonkar V,Qi Z,Tse HF,Lian Q

14-9523 was used in Western Blotting to indicate that Rap1 is essential to maintain the immunomodulatory function of MSCs.

Wed Mar 07 00:00:00 UTC 2018

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma.

Experimental and therapeutic medicine

Nie H,Wang A,He Q,Yang Q,Liu L,Zhang G,Huang Y,Ding X,Yu H,Hu S

14-9523-82 was used in Western Blot to observe the phenotypic characteristics of lung interstitial macrophages in an ovalbumin-induced mouse model of asthma.

Tue Aug 01 00:00:00 UTC 2017

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma.

Experimental and therapeutic medicine

Nie H,Wang A,He Q,Yang Q,Liu L,Zhang G,Huang Y,Ding X,Yu H,Hu S

14-9523-82 was used in Western Blot to observe the phenotypic characteristics of lung interstitial macrophages in an ovalbumin-induced mouse model of asthma.

Tue Aug 01 00:00:00 UTC 2017

The CXCL12/CXCR4 Signaling Pathway: A New Susceptibility Factor in Human Papillomavirus Pathogenesis.

PLoS pathogens

Meuris F,Carthagena L,Jaracz-Ros A,Gaudin F,Cutolo P,Deback C,Xue Y,Thierry F,Doorbar J,Bachelerie F

14-9523 was used in Western Blotting to investigate whether the CXCR4(1013) mutation interferes in the HPV18 life cycle using organotypic epithelial cell cultures.

Thu Dec 01 00:00:00 UTC 2016

Ras-association domain family 10 acts as a novel tumor suppressor through modulating MMP2 in hepatocarcinoma.

Oncogenesis

Liu W,Wang J,Wang L,Qian C,Qian Y,Xuan H,Zhuo W,Li X,Yu J,Si J

Published figure using GAPDH monoclonal antibody (Product # 14-9523-82) in Western Blot

Mon Jun 27 00:00:00 UTC 2016

Inhibition of obesity-induced hepatic ER stress by early insulin therapy in obese diabetic rats.

Endocrine

Sun W,Bi Y,Liang H,Cai M,Chen X,Zhu Y,Li M,Xu F,Yu Q,He X,Ye J,Weng J

14-9523 was used in Immunoprecipitation to suggest that short-term insulin therapy relieves ER stress and enhanced insulin sensitivity in the liver of diabetic rats.

Wed Jun 01 00:00:00 UTC 2011

Inhibition of obesity-induced hepatic ER stress by early insulin therapy in obese diabetic rats.

Endocrine

Sun W,Bi Y,Liang H,Cai M,Chen X,Zhu Y,Li M,Xu F,Yu Q,He X,Ye J,Weng J

14-9523 was used in Immunoprecipitation to suggest that short-term insulin therapy relieves ER stress and enhanced insulin sensitivity in the liver of diabetic rats.

Wed Jun 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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Gene Aliases: CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Mouse) Q64467

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Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Mouse) 14447

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Process(es)

gluconeogenesis spermatid development sperm motility positive regulation of glycolytic process canonical glycolysis glucose metabolic process glycolytic process oxidation-reduction process microtubule cytoskeleton organization regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process cellular response to interferon-gamma carbohydrate metabolic process apoptotic process multicellular organism development response to ammonium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GAPDH Monoclonal Antibody (FF26A), eBioscience™

GAPDH Antibody (14-9523-82) in WB

Product Details

14-9523-82

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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