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GAPDHS Antibody (MA5-33106) in ICC/IF
Immunocytochemistry analysis of GAPDHS in HeLa cells labeled with GAPDHS monoclonal antibody (Product # MA5-33106) using a dilution of 1:100 (green) and nuclear staining with DAPI (blue). The Anti-GAPDH produces strong cytoplasmic labeling of healthy cells.
Product Details
MA5-33106
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
1D4
Immunogen
Purified porcine GAPDH
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802656
Target Information
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 278; GAPDH S; GAPDH-2; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene Aliases: BOS_17541; Gapd-s; GAPD2; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-278; HSD-35; HSD35
UniProt ID: (Human) O14556, (Bovine) Q2KJE5, (Rat) Q9ESV6, (Mouse) Q64467
Entrez Gene ID: (Human) 26330, (Bovine) 532231, (Rat) 66020, (Mouse) 14447
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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