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GAS2 Antibody (MA5-55976) in IHC (P)
Immunohistochemistry analysis of GAS2 in paraffin-embedded liver tissue. Sample was incubated with GAS2 monoclonal antibody (Product # MA5-55976) at a dilution of 10 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly... View More
Product Details
MA5-55976
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K94023_17D9
Immunogen
Recombinant human GAS2 (immunogen range is 11-180).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_3668208
Product Specific Information
Sequence of this protein is as follows: SGPGLSDMHQ YSQWLASRHE ANLLPMKEDL ALWLTNLLGK EITAETFMEK LDNGALLCQL AETMQEKFKE SMDANKPTKN LPLKKIPCKT SAPSGSFFAR DNTANFLSWC RDLGVDETCL FESEGLVLHK QPREVCLCLL ELGRIAARYG VEPPGLIKLE KEIEQEETLS
Target Information
Gas2 is a 313 amino acid protein encoded by the human gene GAS2. Gas2 is thought to play a role in apoptosis by acting as a cell death substrate for caspases. Gas2, a component of the microfilament system, is cleaved by a caspase (caspase-3 and caspase-7) at Asparagine 278 during apoptosis. The cleaved form resulting from this dramatically induces the rearrangement of the Actin cytoskeleton and causes potent changes in the shape of the affected cells. Gas2 is believed to also be involved in the membrane ruffling process. During the G0-G1 transition phase Gas2 can be found phosphorylated on its serine residues. Gas2 is a cytoskeleton and peripheral membrane protein that co-localizes with Actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Gas2 is mainly membrane-associated but when hyperphosphorylated it will accumulate at membrane ruffles. Gas2 is specifically expressed at growth arrest and is ubiquitously expressed with highest levels found in liver, lung and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GAS 2; GAS-2; growth arrest-specific 2; Growth arrest-specific protein 2
Gene Aliases: GAS2
UniProt ID: (Human) O43903
Entrez Gene ID: (Human) 2620
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