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          • Primary Antibodies ›
          • GATA1 Antibodies

          Invitrogen

          GATA1 Polyclonal Antibody

          View all (29) GATA1 antibodies

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          Cite GATA1 Polyclonal Antibody

          • Antibody Testing Data (4)
          GATA1 Antibody in Immunocytochemistry (ICC/IF)
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          GATA1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          GATA1 Antibody (PA5-18408) in ICC/IF

          Immunocytochemistry analysis of GATA1 using GATA1 Polyclonal Antibody (Product # PA5-18408) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GATA1 Antibody in Immunocytochemistry (ICC/IF)
          GATA1 Antibody in Immunocytochemistry (ICC/IF)
          GATA1 Antibody in Western Blot (WB)
          GATA1 Antibody in Flow Cytometry (Flow)

          Product Details

          PA5-18408

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.3-1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          -

          Flow Cytometry (Flow)

          10 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide sequence (DAEAYRHSPVFQ) corresponding to the internal amino acids of GATA1 (aa 65-76).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          TBS, pH 7.3, with 0.5% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10988767

          Product Specific Information

          This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.

          This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

          Target Information

          Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Eryf1; Erythroid transcription factor; erythroid transcription factor 1; GATA binding protein 1 (globin transcription factor 1); GATA-1; GATA-binding factor 1; GATA-binding protein 1; globin transcription factor 1; HGNC:4170; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1

          View more View less

          Gene Aliases: ERYF1; GATA-1; GATA1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT

          View more View less

          UniProt ID: (Human) P15976, (Mouse) P17679

          View more View less

          Entrez Gene ID: (Human) 2623, (Mouse) 14460

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding DNA binding, bending chromatin DNA binding sequence-specific DNA binding C2H2 zinc finger domain binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding metal ion binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling negative regulation of cell proliferation male gonad development regulation of glycoprotein biosynthetic process regulation of definitive erythrocyte differentiation myeloid cell differentiation erythrocyte differentiation megakaryocyte differentiation platelet formation basophil differentiation eosinophil differentiation negative regulation of bone mineralization positive regulation of osteoblast proliferation embryonic hemopoiesis eosinophil fate commitment negative regulation of apoptotic process positive regulation of erythrocyte differentiation positive regulation of transcription from RNA polymerase II promoter cell development erythrocyte development positive regulation of peptidyl-tyrosine phosphorylation platelet aggregation transcriptional activation by promoter-enhancer looping dendritic cell differentiation cellular response to thyroid hormone stimulus negative regulation of transcription regulatory region DNA binding negative regulation of extrinsic apoptotic signaling pathway in absence of ligand heart development blood coagulation organ morphogenesis tissue development regulation of primitive erythrocyte differentiation positive regulation of transcription, DNA-templated digestive tract development homeostasis of number of cells within a tissue
          It has to be done as per old AB suggested Products section.
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