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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GATA3 Antibodies

          OriGene

          GATA3 Monoclonal Antibody (OTI5C11), TrueMAB™

          View all (114) GATA3 antibodies

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          Cite GATA3 Monoclonal Antibody (OTI5C11), TrueMAB™

          • Antibody Testing Data (7)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          GATA3 Antibody (CF809094) in ICC/IF

          Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody (TA809094)(Left). The right is negative control. ((1:2000) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody in Flow Cytometry (Flow)

          Product Details

          CF809094

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200
          -

          Immunocytochemistry (ICC/IF)

          1:200
          -

          Flow Cytometry (Flow)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OTI5C11

          Immunogen

          Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 produced in E.coli.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 8% trehalose

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

          View more View less

          Gene Aliases: Gata-3; GATA3; HDR; HDRS; jal

          View more View less

          UniProt ID: (Human) P23771, (Mouse) P23772

          View more View less

          Entrez Gene ID: (Human) 2625, (Mouse) 14462, (Rat) 85471

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis in utero embryonic development cell fate determination neuron migration cell activation positive regulation of cytokine production kidney development mesonephros development lens development in camera-type eye immune system process pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter humoral immune response signal transduction nervous system development axon guidance negative regulation of cell proliferation male gonad development post-embryonic development positive regulation of signal transduction positive regulation of endothelial cell migration negative regulation of gene expression regulation of neuron projection development phosphatidylinositol 3-kinase signaling neuron differentiation T cell differentiation erythrocyte differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production T cell differentiation in thymus negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process norepinephrine biosynthetic process inner ear morphogenesis regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process ear development thymic T cell selection T-helper 2 cell differentiation innate immune response positive regulation of T cell differentiation positive regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development embryonic organ development developmental growth anatomical structure formation involved in morphogenesis homeostasis of number of cells negative regulation of inflammatory response T cell receptor signaling pathway regulation of histone H3-K4 methylation positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development mesenchymal to epithelial transition mast cell differentiation ureteric bud formation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to cytokine stimulus cellular response to interleukin-4 otic vesicle development cellular response to BMP stimulus renal system development positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation interleukin-4 secretion interferon-gamma secretion lymphocyte migration regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation type IV hypersensitivity response to virus response to gamma radiation cellular response to interferon-alpha response to drug response to estrogen response to ethanol cellular response to tumor necrosis factor chromatin remodeling defense response heart development blood coagulation anatomical structure morphogenesis organ morphogenesis cell fate commitment digestive tract development positive regulation of histone H3-K14 acetylation negative regulation of DNA demethylation positive regulation of T-helper 2 cell cytokine production positive regulation of histone H3-K9 acetylation
          It has to be done as per old AB suggested Products section.
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