OriGene

GATA4 Monoclonal Antibody (OTI9F9), TrueMAB™

View all (28) GATA4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF500091

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

OTI9F9

Immunogen

Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GAT4; GATA-binding factor 4; MGC126629; Transcription factor GATA-4

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Gene Aliases: ASD2; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694

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Entrez Gene ID: (Human) 2626

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding
Process(es)
heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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