Invitrogen

GATA6 Polyclonal Antibody

GATA6 Antibody in Immunocytochemistry (ICC/IF)

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GATA6 Antibody (PA1-104) in ICC/IF

Immunofluorescent analysis of GATA6 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a rabbit polyclonal antibody recognizing GATA6 (Product # PA1-104) at a dilution of 1:200 for at least 1 hour at 37°C. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:200 for 30 ... View More

GATA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA6 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GATA6 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot of GATA6 using anti-GATA6 polyclonal antibody (Product # PA1-104) shows increase in expression of GATA6 upon treatment with 5FU or PTX. {TM}

Product Details

PA1-104

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence KYSGQDGLYIGVSLASPAE & KRFGAAGADASDSRAFPARE

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2539879

Target Information

GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic regulation during cancer transitions across 11 tumour types.

Nature

Terekhanova NV,Karpova A,Liang WW,Strzalkowski A,Chen S,Li Y,Southard-Smith AN,Iglesia MD,Wendl MC,Jayasinghe RG,Liu J,Song Y,Cao S,Houston A,Liu X,Wyczalkowski MA,Lu RJ,Caravan W,Shinkle A,Naser Al Deen N,Herndon JM,Mudd J,Ma C,Sarkar H,Sato K,Ibrahim OM,Mo CK,Chasnoff SE,Porta-Pardo E,Held JM,Pachynski R,Schwarz JK,Gillanders WE,Kim AH,Vij R,DiPersio JF,Puram SV,Chheda MG,Fuh KC,DeNardo DG,Fields RC,Chen F,Raphael BJ,Ding L

PA1-104 was used in Immunohistochemistry to reveal a marked correlation between enhancer accessibility and gene expression and uncovered cooperation between epigenetic and genetic drivers.

Wed Nov 01 00:00:00 UTC 2023

Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.

Nature communications

Xu PF,Borges RM,Fillatre J,de Oliveira-Melo M,Cheng T,Thisse B,Thisse C

PA1-104 was used in Immunohistochemistry to show that embryoids provide an in vitro model of mammalian embryo that displays extensive development of germ layer derivatives and that promises to be a powerful tool for in vitro studies and disease modelling.

Wed Jun 02 00:00:00 UTC 2021

Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.

Nature communications

Xu PF,Borges RM,Fillatre J,de Oliveira-Melo M,Cheng T,Thisse B,Thisse C

Wed Jun 02 00:00:00 UTC 2021

Reproducible differentiation and characterization of neurons from mouse embryonic stem cells.

MethodsX

Saxena S,Choudhury S,Mohan KN

PA1-104 was used in Immunocytochemistry-immunoflourescence to describe a 16-day differentiation protocol that consistently induced differentiation of mESCs into neurons.

Thu Oct 22 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: ASD9; AVSD5; GATA-binding factor 6; GATA-binding protein 6; Transcription factor GATA-6

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Gene Aliases: AA410133; GATA-6; GATA6

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UniProt ID: (Human) Q92908, (Mouse) Q61169

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Entrez Gene ID: (Human) 2627, (Mouse) 14465

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding zinc ion binding protein kinase binding transcription regulatory region DNA binding enhancer sequence-specific DNA binding DNA binding double-stranded DNA binding sequence-specific DNA binding metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GATA6 Polyclonal Antibody

GATA6 Antibody (PA1-104) in ICC/IF

Product Details

PA1-104

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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