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Invitrogen
GCAP1 Monoclonal Antibody (G2)
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Product Details
MA1-724
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Non-human primate
Published species
Bovine,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
G2
Immunogen
Bacterial expressed full length GCAP-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2115533
Product Specific Information
MA1-724 detects GCAP-1 from human, non-human primate and bovine samples. This antibody is specific for GCAP-1 and does not react with other isotypes.
MA1-724 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~23 kDa protein representing GCAP-1 protein on bovine rod outer segment membranes.
The MA1-724 immunogen is bacterial expressed full length GCAP-1.
Target Information
Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cone dystrophy 3; GCAP 1; Guanylate cyclase activator 1A; guanylate cyclase-activating protein, photoreceptor 1; guanylin 1, retina; Guanylyl cyclase-activating protein 1
Gene Aliases: BOS_21468; C6orf131; COD3; CORD14; GCAP; GCAP1; GUCA; GUCA1; GUCA1A
UniProt ID: (Bovine) P46065
Entrez Gene ID: (Bovine) 282243, (Human) 2978
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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