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          • Primary Antibodies ›
          • TCF9 Antibodies

          Bethyl Laboratories

          GCF Polyclonal Antibody

          View all (4) TCF9 antibodies

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          Cite GCF Polyclonal Antibody

          • Antibody Testing Data (1)
          GCF Antibody in Western Blot (WB)
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          GCF Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          GCF Antibody (A301-919A) in WB

          Detection of human and mouse GCF by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GCF antibody (Product # A301-919A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. GCF was also immunoprecipitated by rabbit anti-GCF antibody A301-920A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GCF Antibody in Western Blot (WB)

          Product Details

          A301-919A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-5 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 175 and 225 of human GC-rich sequence DNA-binding factor.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.20 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          TBS, pH 7.0 to 8.0, with 0.1% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Target Information

          TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: GC binding factor; GC bindng factor; GC-rich sequence DNA-binding factor; GC-rich sequence DNA-binding factor 2; GCF; Intron Large complex component GCFC2; TCF-9; Transcription factor 9; transcription factor 9 (binds GC-rich sequences)

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          Gene Aliases: A130099G21; AW146020; C2orf3; DNABF; GCF; GCF2; GCFC2; TCF9

          View more View less

          UniProt ID: (Human) P16383, (Mouse) Q8BKT3

          View more View less

          Entrez Gene ID: (Human) 6936, (Mouse) 330361

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter spliceosomal complex assembly transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing RNA splicing negative regulation of transcription, DNA-templated
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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