Proteintech

GCK Polyclonal Antibody

GCK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GCK Antibody (19666-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver tissue slide using 19666-1-AP ( GCK Antibody) at dilution of 1:200 (under 40x lens).

GCK Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19666-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GCK Fusion Protein Ag8116 (157-465 aa encoded by BC001890)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EDIDKGILL NWTKGFKASG AEGNNVVGLL RDAIKRRGDF EMDVVAMVND TVATMISCYY EDHQCEVGMI VGTGCNACYM EEMQNVELVE GDEGRMCVNT EWGAFGDSGE LDEFLLEYDR LVDESSANPG QQLYEKLIGG KYMGELVRLV LLRLVDENLL FHGEASEQLR TRGAFETRFV SQVESDTGDR KQIYNILSTL GLRPSTTDCD IVRRACESVS TRAAHMCSAG LAGVINRMRE SRSEDVMRIT VGVDGSVYKL HPSFKERFHA SVRRLTPSCE ITFIESEEGS GRGAALVSAV ACKKACMLGQ (157-465 aa encoded by BC001890)

Target Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

β-Cell glucokinase expression was increased in type 2 diabetes subjects with better glycemic control.

Journal of diabetes

Liu J,Fu H,Kang F,Ning G,Ni Q,Wang W,Wang Q

Mon May 01 00:00:00 UTC 2023

Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.

Science translational medicine

Watanabe H,Du W,Son J,Sui L,Asahara SI,Kurland IJ,Kuo T,Kitamoto T,Miyachi Y,de Cabo R,Accili D

Wed Feb 01 00:00:00 UTC 2023

Functionalized gadofullerene ameliorates impaired glycolipid metabolism in type 2 diabetic mice.

Journal of genetics and genomics = Yi chuan xue bao

Wu J,Chen Y,Li X,Ran L,Liu X,Wang X,Zhen M,Shao S,Zeng L,Wang C,Liang B,Zhao J,Wu Y

Fri Apr 01 00:00:00 UTC 2022

Citrullination of glucokinase is linked to autoimmune diabetes.

Nature communications

Yang ML,Horstman S,Gee R,Guyer P,Lam TT,Kanyo J,Perdigoto AL,Speake C,Greenbaum CJ,Callebaut A,Overbergh L,Kibbey RG,Herold KC,James EA,Mamula MJ

Wed Apr 06 00:00:00 UTC 2022

Citrullination of glucokinase is linked to autoimmune diabetes.

Nature communications

Yang ML,Horstman S,Gee R,Guyer P,Lam TT,Kanyo J,Perdigoto AL,Speake C,Greenbaum CJ,Callebaut A,Overbergh L,Kibbey RG,Herold KC,James EA,Mamula MJ

Wed Apr 06 00:00:00 UTC 2022

GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.

The Journal of biological chemistry

Ahat E,Bui S,Zhang J,da Veiga Leprevost F,Sharkey L,Reid W,Nesvizhskii AI,Paulson HL,Wang Y

Mon Aug 01 00:00:00 UTC 2022

Hypoglycemic flavonoids from Selaginella tamariscina (P.Beauv.) Spring.

Phytochemistry

Long HP,Liu J,Xu PS,Xu KP,Li J,Tan GS

Tue Mar 01 00:00:00 UTC 2022

FABP3 Deficiency Exacerbates Metabolic Derangement in Cardiac Hypertrophy and Heart Failure via PPARα Pathway.

Frontiers in cardiovascular medicine

Zhuang L,Mao Y,Liu Z,Li C,Jin Q,Lu L,Tao R,Yan X,Chen K

Tue Aug 31 00:00:00 UTC 2021

Diurnal regulation of oxidative phosphorylation restricts hepatocyte proliferation and inflammation.

Cell reports

Bai X,Liao Y,Sun F,Xiao X,Fu S

Tue Sep 07 00:00:00 UTC 2021

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

PLoS biology

He X,Li M,Yu H,Liu G,Wang N,Yin C,Tu Q,Narla G,Tao Y,Cheng S,Yin H

Tue Dec 01 00:00:00 UTC 2020

Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yang S,Chen Y,Duan Y,Ma C,Liu L,Li Q,Yang J,Li X,Zhao B,Wang Y,Qian K,Liu M,Zhu Y,Yang X,Han J

Tue Oct 01 00:00:00 UTC 2019

Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.

Arthritis research & therapy

Song G,Lu Q,Fan H,Zhang X,Ge L,Tian R,Wang S,Feng T,Pan J,Feng J,Xiao Y,Yi X,Ren N,Wang L

Wed Apr 03 00:00:00 UTC 2019

Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.

Arthritis research & therapy

Song G,Lu Q,Fan H,Zhang X,Ge L,Tian R,Wang S,Feng T,Pan J,Feng J,Xiao Y,Yi X,Ren N,Wang L

Wed Apr 03 00:00:00 UTC 2019

Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.

Toxicology

Wang F,Chang C,Li R,Zhang Z,Jiang H,Zeng N,Li D,Chen L,Xiao Y,Chen W,Wang Q

Thu Aug 01 00:00:00 UTC 2019

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Scientific reports

Yang S,Liu M,Chen Y,Ma C,Liu L,Zhao B,Wang Y,Li X,Zhu Y,Gao X,Kong D,Duan Y,Han J,Yang X

Thu Jun 14 00:00:00 UTC 2018

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Scientific reports

Yang S,Liu M,Chen Y,Ma C,Liu L,Zhao B,Wang Y,Li X,Zhu Y,Gao X,Kong D,Duan Y,Han J,Yang X

Thu Jun 14 00:00:00 UTC 2018

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Free radical biology & medicine

Dong K,Wu M,Liu X,Huang Y,Zhang D,Wang Y,Yan LJ,Shi D

Thu Dec 01 00:00:00 UTC 2016

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Free radical biology & medicine

Dong K,Wu M,Liu X,Huang Y,Zhang D,Wang Y,Yan LJ,Shi D

Thu Dec 01 00:00:00 UTC 2016

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Biochemical and biophysical research communications

Dong K,Ni H,Wu M,Tang Z,Halim M,Shi D

Fri Aug 05 00:00:00 UTC 2016

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Biochemical and biophysical research communications

Dong K,Ni H,Wu M,Tang Z,Halim M,Shi D

Fri Aug 05 00:00:00 UTC 2016

Efficacy of Berberine in Patients with Non-Alcoholic Fatty Liver Disease.

PloS one

Yan HM,Xia MF,Wang Y,Chang XX,Yao XZ,Rao SX,Zeng MS,Tu YF,Feng R,Jia WP,Liu J,Deng W,Jiang JD,Gao X

Sun May 08 00:00:00 UTC 2016

Efficacy of Berberine in Patients with Non-Alcoholic Fatty Liver Disease.

PloS one

Yan HM,Xia MF,Wang Y,Chang XX,Yao XZ,Rao SX,Zeng MS,Tu YF,Feng R,Jia WP,Liu J,Deng W,Jiang JD,Gao X

Sun May 08 00:00:00 UTC 2016

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.

Scientific reports

Liu M,Pan Q,Chen Y,Yang X,Zhao B,Jia L,Zhu Y,Zhang B,Gao X,Li X,Han J,Duan Y

Wed Jun 10 00:00:00 UTC 2015

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.

Scientific reports

Liu M,Pan Q,Chen Y,Yang X,Zhao B,Jia L,Zhu Y,Zhang B,Gao X,Li X,Han J,Duan Y

Wed Jun 10 00:00:00 UTC 2015

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Journal of agricultural and food chemistry

Liu C,Lin JJ,Yang ZY,Tsai CC,Hsu JL,Wu YJ

Wed Dec 03 00:00:00 UTC 2014

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Journal of agricultural and food chemistry

Liu C,Lin JJ,Yang ZY,Tsai CC,Hsu JL,Wu YJ

Wed Dec 03 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: ATP:D-hexose 6-phosphotransferase; GC kinase; Glucokinase; glucokinase (hexokinase 4); glucokinase variant 3; hexokinase 4; hexokinase D, pancreatic isozyme; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; MEKKK 2

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Gene Aliases: FGQTL3; GCK; GK; GLK; Gls006; GLUKA; HHF3; HK4; HKIV; Hlb62; HXKP; LGLK; MODY2; RNGK2

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UniProt ID: (Human) P35557, (Rat) P17712, (Mouse) P52792

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Entrez Gene ID: (Human) 2645, (Rat) 24385, (Mouse) 103988

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Function(s)

magnesium ion binding glucokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity protein phosphatase binding ADP binding nucleotide binding hexokinase activity kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor

Process(es)

cellular glucose homeostasis glycogen biosynthetic process fructose 2,6-bisphosphate metabolic process glucose metabolic process glycolytic process NADP metabolic process positive regulation of cytosolic calcium ion concentration response to glucose second-messenger-mediated signaling positive regulation of insulin secretion negative regulation of epinephrine secretion cellular response to insulin stimulus cellular response to glucose starvation positive regulation of phosphorylation glucose homeostasis regulation of potassium ion transport cellular response to leptin stimulus negative regulation of gluconeogenesis positive regulation of glycogen biosynthetic process positive regulation of glycolytic process carbohydrate phosphorylation regulation of insulin secretion glucose 6-phosphate metabolic process detection of glucose lipid homeostasis calcium ion import regulation of glycolytic process regulation of glucose transport glucose transport canonical glycolysis carbohydrate metabolic process phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GCK Polyclonal Antibody

GCK Antibody (19666-1-AP) in IHC (P)

Product Details

19666-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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