Invitrogen

GCN2 Polyclonal Antibody

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GCN2 Antibody (720463) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HCT116 (Lane 1), HT-29 (Lane 2), HT-1080 (Lane 3), U-87 MG (Lane 4) and HeLa (Lane 5). The blot was probed with Anti-EIF2AK4 Rabbit Polyclonal Antibody (Product # 720463, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). A ~220 kDa band corresponding to EIF2AK4 was observed across the cell lines tested.

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GCN2 Antibody — Antibody specificity was also demonstrated by detection of differential basal expression of the target between cell models owing to their inherent genetic constitution. Higher expression of EIF2AK4 protein was observed in HCT116 in comparison to HT-29 using Anti-EIF2AK4 Rabbit Polyclonal Antibody (Product # 720463, 1:5000 dilution) in western blot. {RE}

Product Details

720463

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Goat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Protein corresponding to human GCN2 [aa136-aa252]

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 30% glycerol, 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2762413

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Bovine, Sheep.

Target Information

EIF2AK4 (GCN2) is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor-2 in response to low amino acid availability. It plays a role as an activator of the integrated stress response required for adapatation to amino acid starvation. Mutations in the gene lead to pulmonary vennocclusive disease 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulation of GCN2/eIF2α/ATF4 Pathway in the Liver and Induction of FGF21 in Young Goats Fed a Protein- and/or Phosphorus-Reduced Diet.

International journal of molecular sciences

Weber SL,Hustedt K,Schnepel N,Visscher C,Muscher-Banse AS

Published figure using GCN2 polyclonal antibody (Product # 720463) in Western Blot

Wed Apr 12 00:00:00 UTC 2023

Modulation of GCN2/eIF2α/ATF4 Pathway in the Liver and Induction of FGF21 in Young Goats Fed a Protein- and/or Phosphorus-Reduced Diet.

International journal of molecular sciences

Weber SL,Hustedt K,Schnepel N,Visscher C,Muscher-Banse AS

Published figure using GCN2 polyclonal antibody (Product # 720463) in Western Blot

Wed Apr 12 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2

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Gene Aliases: EIF2AK4; GCN2; KIAA1338; PVOD2

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UniProt ID: (Human) Q9P2K8

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Entrez Gene ID: (Human) 440275

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Function(s)

tRNA binding protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity ATP binding

Process(es)

positive regulation of defense response to virus by host adaptive immune response T cell activation involved in immune response positive regulation of adaptive immune response regulation of translational initiation protein phosphorylation cell cycle arrest learning long-term memory regulation of translational initiation by eIF2 alpha phosphorylation viral translation negative regulation of translational initiation in response to stress negative regulation of CREB transcription factor activity cellular response to amino acid starvation cellular response to UV eiF2alpha phosphorylation in response to endoplasmic reticulum stress induction by virus of host autophagy negative regulation by host of viral genome replication negative regulation of neuron differentiation negative regulation of translational initiation protein autophosphorylation defense response to virus regulation of feeding behavior regulation of eIF2 alpha phosphorylation by amino acid starvation cellular response to cold positive regulation of translational initiation in response to starvation positive regulation of long-term synaptic potentiation neuron projection extension cellular response to leucine starvation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GCN2 Polyclonal Antibody

GCN2 Antibody (720463) in WB

Product Details

720463

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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