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Invitrogen
GCN4 Chimeric Recombinant Mouse Monoclonal Antibody (C11L34)
FIGURE: 1 / 1
GCN4 Chimeric Antibody (MA5-47957) in ELISA
ELISA analysis of GCN4 recombinant monoclonal antibody (Product # MA5-47957) was performed by coating plate with GCN4 protein at a concentration of 5 µg/mL. The antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.
Product Details
MA5-47957
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Mouse
/ IgG1, lambda
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
C11L34
Immunogen
Biotinylated GCN4-peptide (HLENEVARLKK).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074401
Product Specific Information
Specificity: This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity.
Target Information
GCN4 is a transcription factor protein in yeast that plays a crucial role in the regulation of genes involved in amino acid biosynthesis, such as HIS4, HIS7, HIS3, ARG1, ARG3, ARG4, and ARG5,6. The protein contains a basic leucine zipper (bZIP) domain that enables it to bind to specific DNA sequences, thereby regulating the transcription of target genes. The protein is also involved in the regulation of other cellular processes, including stress response and nutrient signaling, such as GSH1, YAP1, and TRX2. Its structure is composed of a dimeric coiled-coil domain, which allows for protein-protein interactions, and a DNA-binding bZIP domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amino acid biosynthesis regulatory protein; Gcn4p; General control protein GCN4; General control transcription factor GCN4
Gene Aliases: AAS101; AAS3; ARG9; GCN4; YEL009C
UniProt ID: (Baker's yeast) P03069
Entrez Gene ID: (Baker's yeast) 856709
transcription factor activity, TFIID-class binding
transcription factor activity, RNA polymerase II transcription factor recruiting
transcriptional activator activity, RNA polymerase II transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
nitrogen catabolite activation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular amino acid biosynthetic process
negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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