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GDF1 Antibody (PA5-103064) in ICC/IF
Immunocytochemistry analysis of GDF1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GDF1 polyclonal antibody (Product # PA5-103064) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103064
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GDF1(Accession P27539), corresponding to amino acid residues A52-V102.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852434
Product Specific Information
Antibody detects endogenous levels of total GDF1.
Target Information
Growth differentiation factors (GDFs) are members of the transforming growth factor (TGF) superfamily that is involved in embryonic development and adult tissue homeostasis. GDF1 was initially identified as a temporally expressed gene in the mouse central nervous system during embryonic development, with only one isoform detected in adult tissues. GDF1 is required for left-right patterning during development and directly interacts with Nodal, another member of the TGF-beta superfamily. It has been suggested that GDF1 regulates the activity and signaling range of Nodal through direct interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Embryonic growth/differentiation factor 1; GDF-1
Gene Aliases: AI385651; DORV; DTGA3; Gdf-1; GDF1; RAI
UniProt ID: (Human) P27539, (Mouse) P20863
Entrez Gene ID: (Human) 2657, (Mouse) 14559, (Rat) 306351
in utero embryonic development
signal transduction
endoderm development
mesoderm development
positive regulation of pathway-restricted SMAD protein phosphorylation
BMP signaling pathway
regulation of apoptotic process
regulation of MAPK cascade
cell development
SMAD protein signal transduction
growth
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