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GDF10 Antibody (PA5-114912) in ICC/IF
Immunocytochemistry analysis of GDF10 in 3T3 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114912) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114912
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GDF10(Accession P55107), corresponding to amino acid residues T66-F116.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899548
Product Specific Information
Antibody detects endogenous levels of total BMP3B.
Target Information
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BIP; BMP-3B; Bone morphogenetic protein 3B; Bone-inducing protein; GDF-10; Growth/differentiation factor 10
Gene Aliases: BMP-3b; BMP3B; GDF10
UniProt ID: (Human) P55107, (Mouse) P97737
Entrez Gene ID: (Human) 2662, (Mouse) 14560
skeletal system development
osteoblast differentiation
transforming growth factor beta receptor signaling pathway
positive regulation of pathway-restricted SMAD protein phosphorylation
BMP signaling pathway
regulation of apoptotic process
regulation of MAPK cascade
fat cell differentiation
negative regulation of osteoblast differentiation
cell development
SMAD protein signal transduction
positive regulation of osteoblast differentiation
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