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Invitrogen
GDF5 Polyclonal Antibody
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GDF5 Antibody (PA5-47953) in IHC (F)
Immunohistochemical analysis of GDF5 in immersion fixed frozen sections of mouse embyro (12 d.p.c.). Samples were incubated in GDF5 polyclonal antibody (Product # PA5-47953) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-47953
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse GDF-5 Ala376-Arg495
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608779
Product Specific Information
In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF-8, rmGDF-9, recombinant human GDF-11, and rhGDF-15 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BMP-14; Bone morphogenetic protein 14; brachypodism; cartilage-derived morphogenetic protein-1; GDF-5; Growth/differentiation factor 5; H-GDF-5
Gene Aliases: BMP-14; Bmp14; Bp; brp; Cdmp-1; Gdf-5; Gdf5
UniProt ID: (Mouse) P43027
Entrez Gene ID: (Mouse) 14563
chondrocyte differentiation
transmembrane receptor protein serine/threonine kinase signaling pathway
SMAD protein import into nucleus
positive regulation of pathway-restricted SMAD protein phosphorylation
embryonic limb morphogenesis
BMP signaling pathway
positive regulation of BMP signaling pathway
negative regulation of chondrocyte differentiation
positive regulation of chondrocyte differentiation
forelimb morphogenesis
hindlimb morphogenesis
growth
regulation of multicellular organism growth
regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of neuron differentiation
cell development
negative regulation of epithelial cell proliferation
cartilage development
SMAD protein signal transduction
chondroblast differentiation
mitophagy in response to mitochondrial depolarization
negative regulation of mesenchymal cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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