Invitrogen

GDI1 Polyclonal Antibody

4 Published Figures

15 References

View all (23) GDI1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (4)

FIGURE: 1 / 6

GDI1 Antibody (71-0300) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of IMR32 (Lane 1), T98G (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HEK-293 (Lane 5).The blots were probed with GDI1 Rabbit polyclonal Antibody (Product # 71-0300, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~55 kDa band corresponding to GDI1 was observed across the cell lines tested. Known quantity of protein samples we... View More

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Product Details

71-0300

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 15 publications 15 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 20 amino acid synthetic peptide derived from bovine brain Rab Guanine Nucleotide Dissociation Inhibitor (GDI).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533970

Target Information

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diets Differently Regulate Pulmonary Pathogenesis and Immune Signaling in Mice during Acute and Chronic Mycobacterium tuberculosis Infection.

Life (Basel, Switzerland)

Oswal N,Thangavel H,Lizardo K,Dhanyalayam D,Sidrat T,Salgame P,Nagajyothi JF

71-0300 was used in Western Blotting to suggest that slight adiposity likely protects the host during active TB infection by regulating immune and metabolic conditions in the lungs.

Fri Jan 13 00:00:00 UTC 2023

Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice.

Frontiers in cardiovascular medicine

Dhanyalayam D,Thangavel H,Lizardo K,Oswal N,Dolgov E,Perlin DS,Nagajyothi JF

71-0300 was used in Western Blotting to conclude that the C-ApN/AMPK and b-AR/PPAR downstream signaling may play major roles in determining the progression, severity, and phenotype of CCM and heart failure in the context of COVID.

Tue Nov 15 00:00:00 UTC 2022

Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse.

Metabolism: clinical and experimental

D'Adamo P,Horvat A,Gurgone A,Mignogna ML,Bianchi V,Masetti M,Ripamonti M,Taverna S,Velebit J,Malnar M,Muhič M,Fink K,Bachi A,Restuccia U,Belloli S,Moresco RM,Mercalli A,Piemonti L,Potokar M,Bobnar ST,Kreft M,Chowdhury HH,Stenovec M,Vardjan N,Zorec R

71-0300 was used in Western Blot to reveal a new astrocyte-based mechanism in neurodevelopmental disorders and open a novel therapeutic opportunity of targeting aerobic glycolysis.

Mon Mar 01 00:00:00 UTC 2021

High fat diet aggravates cardiomyopathy in murine chronic Chagas disease.

Microbes and infection

Lizardo K,Ayyappan JP,Cui MH,Balasubramanya R,Jelicks LA,Nagajyothi JF

71-0300 was used in Western Blotting to show that HFD perturbs lipid metabolism and induces oxidative stress to exacerbate late chronic Chagas disease cardiac pathology.

Thu Apr 11 00:00:00 UTC 2019

Inhibition of ER Stress by 2-Aminopurine Treatment Modulates Cardiomyopathy in a Murine Chronic Chagas Disease Model.

Biomolecules & therapeutics

Ayyappan JP,Lizardo K,Wang S,Yurkow E,Nagajyothi JF

71-0300 was used in Western Blotting to demonstrate that inhibition of ER stress improves cardiac pathology caused by T. cruzi infection by reducing ER stress and downstream signaling of phosphorylated eukaryotic initiation factor (P-elF2α) in the hearts of chronically infected mice.

Mon Jul 01 00:00:00 UTC 2019

Adipose Tissue Regulates Pulmonary Pathology during TB Infection.

mBio

Ayyappan JP,Ganapathi U,Lizardo K,Vinnard C,Subbian S,Perlin DS,Nagajyothi JF

71-0300 was used in Western Blotting to investigate the association between Mycobacterium tuberculosis infection, adipose tissue, and tuberculosis disease progression.

Tue Apr 16 00:00:00 UTC 2019

Effect of Mycobacterium tuberculosis infection on adipocyte physiology.

Microbes and infection

Ayyappan JP,Vinnard C,Subbian S,Nagajyothi JF

71-0300 was used in Western Blotting to better understand the mechanisms behind bacterial persistence and the establishment of latency.

Thu Feb 01 00:00:00 UTC 2018

Diet regulates liver autophagy differentially in murine acute Trypanosoma cruzi infection.

Parasitology research

Lizardo K,Almonte V,Law C,Aiyyappan JP,Cui MH,Nagajyothi JF

710300 was used in western blot to analyze the effect of a high-fat diet on liver morphology and physiology, lipid metabolism, immune signaling, energy homeostasis, and stress responses during acute Trypanosoma cruzi infection

Wed Feb 01 00:00:00 UTC 2017

Antagonistic effect of atorvastatin on high fat diet induced survival during acute Chagas disease.

Microbes and infection

Zhao D,Lizardo K,Cui MH,Ambadipudi K,Lora J,Jelicks LA,Nagajyothi JF

710300 was used in western blot to assess the effects of statins on Trypanosoma cruzi infection-induced myocarditis

Tue Nov 01 00:00:00 UTC 2016

The transient expression of Klf4 and Klf5 during adipogenesis depends on GSK3β activity.

Adipocyte

Cervantes-Camacho C,Beltrán-Langarica A,Ochoa-Uribe AK,Marsch-Moreno M,Ayala-Sumuano JT,Velez-delValle C,Kuri-Harcuch W

71-0300 was used in Western Blotting to show that GSK3Β activity was required for Klf4 and Klf5 expression during adipogenesis.

Fri Oct 09 00:00:00 UTC 2015

High fat diet modulates Trypanosoma cruzi infection associated myocarditis.

PLoS neglected tropical diseases

Nagajyothi F,Weiss LM,Zhao D,Koba W,Jelicks LA,Cui MH,Factor SM,Scherer PE,Tanowitz HB

71-0300 was used in western blot to determine the effect of high fat diet on regulating acute T. cruzi infection-induced myocarditis

Wed Oct 01 00:00:00 UTC 2014

EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells.

The Journal of biological chemistry

Imai A,Yoshie S,Ishibashi K,Haga-Tsujimura M,Nashida T,Shimomura H,Fukuda M

Fri Sep 30 00:00:00 UTC 2011

Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells.

Molecular biology of the cell

Kuliawat R,Kalinina E,Bock J,Fricker L,McGraw TE,Kim SR,Zhong J,Scheller R,Arvan P

Thu Apr 01 00:00:00 UTC 2004

Deletion of the mental retardation gene Gdi1 impairs associative memory and alters social behavior in mice.

Human molecular genetics

D'Adamo P,Welzl H,Papadimitriou S,Raffaele di Barletta M,Tiveron C,Tatangelo L,Pozzi L,Chapman PF,Knevett SG,Ramsay MF,Valtorta F,Leoni C,Menegon A,Wolfer DP,Lipp HP,Toniolo D

Tue Oct 01 00:00:00 UTC 2002

Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.

The Journal of biological chemistry

Greenfield JP,Leung LW,Cai D,Kaasik K,Gross RS,Rodriguez-Boulan E,Greengard P,Xu H

Fri Apr 05 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: GDI-1; GDP-dissociation inhibitor 1; Guanosine diphosphate dissociation inhibitor 1; mental retardation, X-linked 48; Oligophrenin-2; Protein XAP-4; Rab GDI alpha; Rab GDIalpha; Rab GDP dissociation inhibitor alpha; rab GDP-dissociation inhibitor, alpha; RABGD RABGDIA; SMG p25A GDI; testis secretory sperm-binding protein Li 208a

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Gene Aliases: 1A; BOS_25443; GDI1; GDIA; GDIalpha; GDIL; MRX41; MRX48; OPHN2; RABGD1A; RABGDIA; XAP-4; XAP4

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UniProt ID: (Bovine) P21856, (Human) P31150, (Mouse) P50396, (Rat) P50398

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Entrez Gene ID: (Bovine) 281188, (Human) 2664, (Mouse) 14567, (Rat) 25183

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Function(s)

Rab GDP-dissociation inhibitor activity GTPase activator activity GDP-dissociation inhibitor activity protein binding oxidoreductase activity Rab GTPase binding

Process(es)

small GTPase mediated signal transduction protein transport negative regulation of axonogenesis signal transduction Rab protein signal transduction positive regulation of GTPase activity regulation of small GTPase mediated signal transduction response to calcium ion oxidation-reduction process negative regulation of protein targeting to membrane regulation of catalytic activity

Performance Guarantee

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GDI1 Polyclonal Antibody

GDI1 Antibody (71-0300) in WB

Product Details

71-0300

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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