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GDNF Antibody (711074) in ICC/IF
For immunofluorescence analysis, T98G glioblastoma cells were fixed and permeabilized for detection of endogenous GDNF using Anti- GDNF Recombinant Rabbit Superclonal™ Antibody (Product # 711074, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of GDNF protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Pro... View More
Product Details
711074
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
13HCLC
Immunogen
Protein corresponding to Human GDNF (aa 78-211)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633113
Product Specific Information
This antibody is predicted to react with Monkey, Horse and Pig
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte-derived trophic factor; ATF; Glial cell line-derived neurotrophic factor; H-GDNF; hGDNF
Gene Aliases: ATF1; ATF2; GDNF; HFB1-GDNF; HSCR3
UniProt ID: (Human) P39905
Entrez Gene ID: (Human) 2668
MAPK cascade
metanephros development
branching involved in ureteric bud morphogenesis
neural crest cell migration
organ induction
postsynaptic membrane organization
mesenchymal to epithelial transition involved in metanephros morphogenesis
signal transduction
nervous system development
positive regulation of cell proliferation
adult locomotory behavior
regulation of gene expression
postganglionic parasympathetic fiber development
peristalsis
neuron projection development
positive regulation of monooxygenase activity
positive regulation of dopamine secretion
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mRNA stabilization
enteric nervous system development
sympathetic nervous system development
regulation of dopamine uptake involved in synaptic transmission
ureteric bud formation
regulation of morphogenesis of a branching structure
positive regulation of ureteric bud formation
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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