Invitrogen

GEF-H1 Monoclonal Antibody (K.607.1)

GEF-H1 Antibody in Immunoprecipitation (IP)

FIGURE: 1 / 1

GEF-H1 Antibody (MA5-15027) in IP

Immunoprecipitation of GEF-H1 from HeLa cell extracts using a GEF-H1 monoclonal antibody (Product # MA5-15027) followed by Western blot analysis using the same antibody.

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Product Details

MA5-15027

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.607.1

Immunogen

Synthetic peptide corresponding to the sequence of human GEF-H1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985904

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

GEF-H1 activates Rho GTPases by promoting the exchange of GDP and GTP. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. GEF-H1 may be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Regulatory Subunit PPP2R2A of PP2A Enhances Th1 and Th17 Differentiation through Activation of the GEF-H1/RhoA/ROCK Signaling Pathway.

Journal of immunology (Baltimore, Md. : 1950)

Pan W,Nagpal K,Suárez-Fueyo A,Ferretti A,Yoshida N,Tsokos MG,Tsokos GC

MA5-15027 was used in Western Blotting to indicate that PPP2R2A is the regulatory subunit that dictates the PP2A-directed enhanced Th1 and Th17 differentiation, and therefore, it represents a therapeutic target for pathologies linked to Th1 and Th17 cell expansion.

Thu Apr 15 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: DKFZp547L106; DKFZp547P1516; FLJ45440; GEF-H1; Guanine nucleotide exchange factor H1; LBC'S first cousin; Lymphoid blast crisis-like 1; Microtubule-regulated Rho-GEF; Oncogene LFC; OTTHUMP00000018855; Proliferating cell nucleolar antigen p40; Rho guanine nucleotide exchange factor 2; rho guanine nucleotide exchange factor 2-like protein; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rhobin

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Gene Aliases: AA408978; ARHGEF2; GEF; GEF-H1; GEFH1; KIAA0651; Lbcl1; Lfc; LFP40; mKIAA0651; P40

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UniProt ID: (Human) D3DVA6, (Mouse) Q60875, (Rat) Q5FVC2

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Entrez Gene ID: (Human) 9181, (Chinese hamster) 100758849, (Mouse) 16800, (Rat) 310635

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Function(s)

guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity protein binding microtubule binding transcription factor binding zinc ion binding Rho GTPase binding Rac guanyl-nucleotide exchange factor activity Rac GTPase binding metal ion binding

Process(es)

establishment of mitotic spindle orientation cell morphogenesis intracellular protein transport actin filament organization negative regulation of microtubule depolymerization mitotic nuclear division positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production regulation of Rho protein signal transduction intracellular signal transduction regulation of cell proliferation positive regulation of apoptotic process positive regulation of GTPase activity innate immune response positive regulation of transcription from RNA polymerase II promoter positive regulation of peptidyl-tyrosine phosphorylation negative regulation of neurogenesis regulation of small GTPase mediated signal transduction positive regulation of NF-kappaB transcription factor activity cell division negative regulation of necroptotic process cellular response to muramyl dipeptide cellular response to tumor necrosis factor cellular hyperosmotic response negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress immune system process cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GEF-H1 Monoclonal Antibody (K.607.1)

GEF-H1 Antibody (MA5-15027) in IP

Product Details

MA5-15027

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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