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Invitrogen
GEN1 Polyclonal Antibody
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GEN1 Antibody (PA5-26502) in WB
Western blot analysis of GEN1 in various lysates. Samples were incubated with GEN1 polyclonal antibody (Product # PA5-26502) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: HL-60 whole cell lysate; Lane 2: K562 whole cell lysate; Lane 3: MCF-7 whole cell lysate. Predicted band size: 103 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-26502
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human GEN1
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544002
Target Information
Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Flap endonuclease GEN homolog 1; Gen endonuclease homolog 1; Gen homolog 1, endonuclease; GEN1 Holliday junction 5' flap endonuclease; Holliday junction resolvase
Gene Aliases: 5830483C08Rik; Gen; GEN1
UniProt ID: (Human) Q17RS7, (Mouse) Q8BMI4
Entrez Gene ID: (Human) 348654, (Mouse) 209334
DNA binding
endodeoxyribonuclease activity
crossover junction endodeoxyribonuclease activity
metal ion binding
catalytic activity
nuclease activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
double-strand break repair via homologous recombination
regulation of centrosome duplication
resolution of recombination intermediates
resolution of mitotic recombination intermediates
positive regulation of mitotic cell cycle spindle assembly checkpoint
nucleic acid phosphodiester bond hydrolysis
DNA repair
cellular response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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