Invitrogen

GFAP Polyclonal Antibody

GFAP Antibody in Immunocytochemistry (ICC/IF)

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GFAP Antibody (PA3-16727) in ICC/IF

Immunocytochemistry analysis of GFAP in Rat neurons. Samples were incubated in GFAP polyclonal antibody (Product # PA3-16727). Neurofilament Heavy antibody (red) and GFAP antibody (green).

GFAP Antibody in Immunohistochemistry - Free Floating (IHC (Free))

GFAP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GFAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of GFAP was observed in Mouse brain, Rat brain in comparison to Mouse liver and Rat liver using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA3-16727) in Western Blot. {RE}

Product Details

PA3-16727

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Immunohistochemistry - Free Floating (IHC (Free))

1:5,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000-1:5,000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Horse, Guinea pig, Human, Mouse, Pig, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full-length human GFAP isotype 1 expressed in and purified from E. coli

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2109795

Product Specific Information

This antibody is likely to react with most mammals.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation.

Cells

Silva EA,Dalla Costa AP,Ruas JS,Siqueira-Santos ES,Francisco A,Castilho RF

PA3-16727 was used in Immunocytochemistry-immunofluorescence to suggest that astrocytes in primary culture can sustainably proliferate when their energy metabolism relies only on aerobic glycolysis since their growth and survival do not require electron flux through respiratory complex I or oxidative phosphorylation.

Tue Feb 21 00:00:00 UTC 2023

Cellular Sources and Neuroprotective Roles of Interleukin-10 in the Facial Motor Nucleus after Axotomy.

Cells

Runge EM,Setter DO,Iyer AK,Regele EJ,Kennedy FM,Sanders VM,Jones KJ

PA3-16727 was used in Immunohistochemistry to suggest that coordinated neuronal and astrocytic IL-10 production is necessary for FMN survival and has roles in neuronal homeostasis, as well as neuroprotective trophism after axotomy.

Sun Oct 09 00:00:00 UTC 2022

The mitochondria-targeted antioxidant MitoQ inhibits memory loss, neuropathology, and extends lifespan in aged 3xTg-AD mice.

Molecular and cellular neurosciences

Young ML,Franklin JL

PA3-16727 was used in Western Blotting to support a role for the involvement of mitochondria-derived oxidative stress in the etiology of AD and suggest that mitochondria-targeted antioxidants may lessen symptoms in AD patients.

Sun Dec 01 00:00:00 UTC 2019

Statins impact primary embryonic mouse neural stem cell survival, cell death, and fate through distinct mechanisms.

PloS one

Carson RA,Rudine AC,Tally SJ,Franks AL,Frahm KA,Waldman JK,Silswal N,Burale S,Phan JV,Chandran UR,Monaghan AP,DeFranco DB

PA3-16727 was used in Immunocytochemistry-immunoflourescence to study the effect of statins on the developing brain.

Mon Aug 06 00:00:00 UTC 2018

Statins impact primary embryonic mouse neural stem cell survival, cell death, and fate through distinct mechanisms.

PloS one

Carson RA,Rudine AC,Tally SJ,Franks AL,Frahm KA,Waldman JK,Silswal N,Burale S,Phan JV,Chandran UR,Monaghan AP,DeFranco DB

PA3-16727 was used in Immunocytochemistry-immunoflourescence to study the effect of statins on the developing brain.

Mon Aug 06 00:00:00 UTC 2018

Involvement of aberrant cyclin-dependent kinase 5/p25 activity in experimental traumatic brain injury.

Journal of neurochemistry

Yousuf MA,Tan C,Torres-Altoro MI,Lu FM,Plautz E,Zhang S,Takahashi M,Hernandez A,Kernie SG,Plattner F,Bibb JA

PA3-16727 was used in immunohistochemistry - paraffin section and western blot to study experimental traumatic brain injury and the role of aberrant Cdk5/p25 activity

Fri Jul 01 00:00:00 UTC 2016

Involvement of aberrant cyclin-dependent kinase 5/p25 activity in experimental traumatic brain injury.

Journal of neurochemistry

Yousuf MA,Tan C,Torres-Altoro MI,Lu FM,Plautz E,Zhang S,Takahashi M,Hernandez A,Kernie SG,Plattner F,Bibb JA

PA3-16727 was used in immunohistochemistry - paraffin section and western blot to study experimental traumatic brain injury and the role of aberrant Cdk5/p25 activity

Fri Jul 01 00:00:00 UTC 2016

Optimizing neuronal differentiation of human pluripotent NT2 stem cells in monolayer cultures.

Development, growth & differentiation

Abolpour Mofrad S,Kuenzel K,Friedrich O,Gilbert DF

PA316727 was used in immunocytochemistry to optimize the differentiation of human pluripotent embryonal carcinoma cells

Sat Oct 01 00:00:00 UTC 2016

Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia.

Biochimica et biophysica acta

Romero JI,Hanschmann EM,Gellert M,Eitner S,Holubiec MI,Blanco-Calvo E,Lillig CH,Capani F

PA3-16727 was used in western blot to examine the role of Trx family proteins in neuronal development and recovery after hypoxia/ischemia and reperfusion.

Mon Jun 01 00:00:00 UTC 2015

Oral administration of the p38α MAPK inhibitor, UR13870, inhibits affective pain behavior after spinal cord injury.

Pain

Galan-Arriero I,Avila-Martin G,Ferrer-Donato A,Gomez-Soriano J,Bravo-Esteban E,Taylor J

PA3-16727 was used in immunohistochemistry - free floating to test the effect of a p38alpha MAPK inhibitor on spinal cord injury in the rat

Wed Oct 01 00:00:00 UTC 2014

Identification of a dithiol-disulfide switch in collapsin response mediator protein 2 (CRMP2) that is toggled in a model of neuronal differentiation.

The Journal of biological chemistry

Gellert M,Venz S,Mitlöhner J,Cott C,Hanschmann EM,Lillig CH

PA3-16727 was used in western blot to study the role of a CRMP2 dithiol-disulfide molecular switch in the mechanism by which Grx2c modulates neuronal differentiation

Fri Dec 06 00:00:00 UTC 2013

Identification of a dithiol-disulfide switch in collapsin response mediator protein 2 (CRMP2) that is toggled in a model of neuronal differentiation.

The Journal of biological chemistry

Gellert M,Venz S,Mitlöhner J,Cott C,Hanschmann EM,Lillig CH

PA3-16727 was used in western blot to study the role of a CRMP2 dithiol-disulfide molecular switch in the mechanism by which Grx2c modulates neuronal differentiation

Fri Dec 06 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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Gene Aliases: AI836096; ALXDRD; BOS_19250; GFAP

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UniProt ID: (Bovine) Q28115, (Human) P14136, (Rat) P47819, (Mouse) P03995

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Entrez Gene ID: (Bovine) 281189, (Horse) 100033970, (Rabbit) 100352532, (Pig) 396562, (Human) 2670, (Rat) 24387, (Mouse) 14580

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Function(s)

structural molecule activity glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding

Process(es)

positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation long-term synaptic potentiation response to wounding intermediate filament-based process positive regulation of glial cell proliferation regulation of protein complex assembly regulation of chaperone-mediated autophagy

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GFAP Polyclonal Antibody

GFAP Antibody (PA3-16727) in ICC/IF

Product Details

PA3-16727

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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