Invitrogen

GFAP Polyclonal Antibody

GFAP Antibody in Immunocytochemistry (ICC/IF)

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GFAP Antibody (PA5-85109) in ICC/IF

GFAP Polyclonal Antibody detects GFAP protein at glia cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFAP protein stained by GFAP Polyclonal Antibody (Product # PA5-85109) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

PA5-85109

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

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Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2792257

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (94%), Rat (94%), Pig (95%), Bovine (97%).

Positive Control: U87-MG, mouse brain, rat brain, GFAP-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Real-Time Noninvasive Bioluminescence, Ultrasound and Photoacoustic Imaging in NFκB-RE-Luc Transgenic Mice Reveal Glia Maturation Factor-Mediated Immediate and Sustained Spatio-Temporal Activation of NFκB Signaling Post-Traumatic Brain Injury in a Gender-Specific Manner.

Cellular and molecular neurobiology

Raikwar SP,Thangavel R,Ahmed ME,Selvakumar GP,Kempuraj D,Wu K,Khan O,Bazley K,Bussinger B,Kukulka K,Zaheer S,Iyer SS,Govindarajan R,Burton C,James D,Zaheer A

PA5-85109 was used in Immunohistochemistry-immunofluorescence to investigate if real-time noninvasive bioluminescence molecular imaging allows rapid and precise monitoring of traumatic brain injury-induced immediate and rapid spatio-temporal activation of NFκB signaling pathway in response to Glia maturation factor (GMF) upregulation which in turn leads to neuroinflammation and neurodegeneration post-TBI.

Mon Nov 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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Gene Aliases: AI836096; ALXDRD; GFAP

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UniProt ID: (Human) P14136, (Rat) P47819, (Mouse) P03995

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Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580

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Function(s)

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

Process(es)

response to wounding positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament-based process intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation positive regulation of glial cell proliferation long-term synaptic potentiation regulation of protein complex assembly regulation of chaperone-mediated autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GFAP Polyclonal Antibody

GFAP Antibody (PA5-85109) in ICC/IF

Product Details

PA5-85109

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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