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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GFAP Antibodies

          Invitrogen

          GFAP Monoclonal Antibody (GA5), Alexa Fluor™ 488, eBioscience™

          Advanced Verification
          36 Published Figures
          54 References
          View all (235) GFAP antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite GFAP Monoclonal Antibody (GA5), Alexa Fluor™ 488, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (36)
          • Advanced Verification (1)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 38

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          GFAP Antibody (53-9892-82) in ICC/IF

          Immunocytochemistry of fixed and permeabilized C6 cells (left) or immunohistochemistry of formalin-fixed paraffin embedded human cerebellum (right) using 10 µg/mL Anti-GFAP Alexa Fluor® 488. Nuclei were counterstained with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          GFAP Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunocytochemistry (ICC/IF)
          GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))
          GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Immunohistochemistry (IHC)
          GFAP Antibody in Western Blot (WB)
          GFAP Antibody in Western Blot (WB)
          GFAP Antibody in Western Blot (WB)
          GFAP Antibody in Flow Cytometry (Flow)
          GFAP Antibody in Flow Cytometry (Flow)
          GFAP Antibody in Flow Cytometry (Flow)
          GFAP Antibody in Flow Cytometry (Flow)
          GFAP Antibody

          Product Details

          53-9892-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 35 publications 35 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          10 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          10 µg/mL
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          View 8 publications 8 publications

          Flow Cytometry (Flow)

          Assay-Dependent
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Pig, Rabbit, Rat

          Published species

          Human, Mouse, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          GA5

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • eFluor 570
          • eFluor 615
          • eFluor 660
          • Request custom conjugation

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10598515

          Product Specific Information

          Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.

          Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistology staining of frozen and FFPE tissue sections, and immunocytochemistry.

          Applications Tested: This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized C6 cells and by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

          View more View less

          Gene Aliases: AI836096; ALXDRD; GFAP

          View more View less

          UniProt ID: (Human) P14136, (Rat) P47819, (Mouse) P03995

          View more View less

          Entrez Gene ID: (Rabbit) 100352532, (Pig) 396562, (Human) 2670, (Rat) 24387, (Mouse) 14580

          View more View less

          Function(s)
          glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity
          Process(es)
          response to wounding positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament-based process intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation positive regulation of glial cell proliferation long-term synaptic potentiation regulation of protein complex assembly regulation of chaperone-mediated autophagy
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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