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FIGURE: 1 / 1
GGH Antibody (PA5-96332) in WB
Western blot analysis of GGH in extracts of various cell lines. Samples were incubated with GGH Polyclonal antibody (Product # PA5-96332) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96332
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-230 of human GGH (NP_0038691)
Conjugate
Form
Liquid
Concentration
0.44 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808134
Product Specific Information
Immunogen sequence: RPHGDTAKKP IIGILMQKCR NKVMKNYGRY YIAASYVKYL ESAGARVVPV RLDLTEKDYE ILFKSINGIL FPGGSVDLRR SDYAKVAKIF YNLSIQSFDD GDYFPVWGTC LGFEELSLLI SGECLLTATD TVDVAMPLNF TGGQLHSRMF QNFPTELLLS LAVEPLTANF HKWSLSVKNF TMNEKLKKFF NVLTTNTDGK IEFIST; Positive Samples: HeLa, Mouse kidney; Cellular Location: Lysosome, Melanosome, extracellular space
Target Information
Gamma-Glutamyl hydrolase (GGH) is a homodimeric protein that catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates and is a central enzyme in folyl and antifolyl poly-gamma-glutamate metabolism. The activities of the baculovirus-expressed enzyme and the enzyme from HL-60 cells were similar on methotrexate polyglutamates. GGH is expressed in a variety of human tumors and correlates with poor prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Conjugase; FGPH; Folylpolyglutamate hydrolase; Gamma-Glu-X carboxypeptidase; Gamma-glutamyl hydrolase; gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase); GH
Gene Aliases: gamma-GH; GGH; GH
UniProt ID: (Human) Q92820, (Mouse) Q9Z0L8
Entrez Gene ID: (Human) 8836, (Mouse) 14590
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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