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Invitrogen
GIF Recombinant Rabbit Monoclonal Antibody (012)
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GIF Antibody (MA5-29292) in WB
Western Blot using GIF Recombinant Rabbit Monoclonal Antibody (12) (Product # MA5-29292) at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.
Product Details
MA5-29292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
012
Immunogen
Recombinant Human Gastric intrinsic factor/GIF Protein (Met1-Tyr417)
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785185
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Gastric intrinsic factor/GIF.
Target Information
Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cobalamin binding intrinsic factor; Gastric intrinsic factor; gastric intrinsic factor (vitamin B synthesis); IF; Intrinsic factor
Gene Aliases: CBLIF; GIF; IF; IFMH; INF; TCN3
UniProt ID: (Human) P27352
Entrez Gene ID: (Human) 2694
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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