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Invitrogen
GLAST Polyclonal Antibody
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FIGURE: 1 / 1
GLAST Antibody (PA5-90649) in WB
Western blot analysis of GLAST in extracts of various cell lines. Samples were incubated with GLAST Polyclonal antibody (Product # PA5-90649) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 453-542 of human EAAT1/EAAT1/SLC1A3 (NP_0041633)
Conjugate
Form
Liquid
Concentration
0.87 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806161
Product Specific Information
Immunogen sequence: IVLTSVGLPT DDITLIIAVD WFLDRLRTTT NVLGDSLGAG IVEHLSRHEL KNRDVEMGNS VIEENEMKKP YQLIAQDNET EKPIDSETKM; Positive Samples: 293T, U-251MG, HeLa, H460, C6, Rat lung; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
This gene encodes a member of a member of a high affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EAAT-1; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST-1; Glial glutamate transporter; Glial high affinity glutamate transporter; GluT-1; glutamate transporter; glutamate/aspartate transporter; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Solute carrier family 1 member 3; solute carrier family 1, member 3
Gene Aliases: AI504299; B430115D02Rik; EA6; EAAT1; GLAST; GLAST-1; GLAST1; GLU-T; GluT-1; Gmt1; MGluT1; SLC1A3
UniProt ID: (Human) P43003, (Rat) P24942, (Mouse) P56564
Entrez Gene ID: (Human) 6507, (Rat) 29483, (Mouse) 20512
neurotransmitter uptake
positive regulation of defense response to virus by host
amino acid transmembrane transport
glutamate biosynthetic process
ion transport
chemical synaptic transmission
sensory perception of sound
response to light stimulus
gamma-aminobutyric acid biosynthetic process
response to wounding
glutamate secretion
cranial nerve development
auditory behavior
response to drug
malate-aspartate shuttle
response to antibiotic
cell morphogenesis involved in neuron differentiation
positive regulation of synaptic transmission
neuromuscular process controlling balance
L-glutamate import
D-aspartate import
L-glutamate transmembrane transport
mitophagy in response to mitochondrial depolarization
xenophagy
glutamate metabolic process
neurotransmitter transport
response to inorganic substance
L-glutamate transport
sodium ion transmembrane transport
transport
dicarboxylic acid transport
response to amino acid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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