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          • Primary Antibodies ›
          • GLI3 Antibodies

          Invitrogen

          GLI3 Polyclonal Antibody

          View all (12) GLI3 antibodies

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          Cite GLI3 Polyclonal Antibody

          • Antibody Testing Data (1)
          GLI3 Antibody in Western Blot (WB)
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          GLI3 Antibody in Western Blot (WB)
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          GLI3 Antibody (PA5-95052) in WB

          Western blot analysis of GLI3 in Lane 1: rat testis tissue lysate Lane 2: A549 cell lysate Lane 3: SW620 cell lysate. Samples were incubated with GLI3 polyclonal antibody (Product # PA5-95052). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GLI3 Antibody in Western Blot (WB)

          Product Details

          PA5-95052

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3 (40-57aa SNEDESPGQTYHRERRNA).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 5mg BSA

          Contains

          0.05mg sodium azide, 0.05mg thimerosal

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806858

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: Rat Testis Tissue, A549 Cell, SW620 Cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: anterior digit pattern deformity; brachyphalangy; extra toes; GLI-Kruppel family member GLI3; GLI3 form of 190 kDa; GLI3 full length protein; GLI3 full-length protein; GLI3-190; GLI3FL; glioma-associated oncogene family zinc finger 3; oncogene GLI3; polydactyly Nagoya; Transcriptional activator GLI3; zinc finger protein GLI3

          View more View less

          Gene Aliases: ACLS; add; AI854843; AU023367; Bph; GCPS; GLI3; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; Pdn; PHS; PPDIV; Xt

          View more View less

          UniProt ID: (Human) P10071, (Mouse) Q61602

          View more View less

          Entrez Gene ID: (Human) 2737, (Rat) 140588, (Mouse) 14634

          View more View less

          Function(s)
          RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding histone acetyltransferase binding histone deacetylase binding metal ion binding nucleic acid binding DNA binding sequence-specific DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter metanephros development branching involved in ureteric bud morphogenesis in utero embryonic development positive regulation of neuroblast proliferation transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance hindgut morphogenesis heart development negative regulation of cell proliferation anterior/posterior pattern specification proximal/distal pattern formation protein processing optic nerve morphogenesis hippocampus development smoothened signaling pathway involved in ventral spinal cord interneuron specification smoothened signaling pathway involved in spinal cord motor neuron cell fate specification forebrain dorsal/ventral pattern formation layer formation in cerebral cortex forebrain radial glial cell differentiation lateral ganglionic eminence cell proliferation melanocyte differentiation lung development positive regulation of chondrocyte differentiation T cell differentiation in thymus limb morphogenesis wound healing positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth embryonic digit morphogenesis negative regulation of apoptotic process nose morphogenesis tongue development response to estrogen negative thymic T cell selection negative regulation of neuron differentiation positive regulation of osteoblast differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation embryonic digestive tract morphogenesis embryonic digestive tract development developmental growth camera-type eye morphogenesis embryonic skeletal system morphogenesis oligodendrocyte differentiation palate development frontal suture morphogenesis lambdoid suture morphogenesis sagittal suture morphogenesis mammary gland specification smoothened signaling pathway involved in dorsal/ventral neural tube patterning artery development anterior semicircular canal development lateral semicircular canal development cell differentiation involved in kidney development thymocyte apoptotic process negative regulation of canonical Wnt signaling pathway regulation of bone development kidney development transcription, DNA-templated regulation of transcription, DNA-templated pattern specification process central nervous system development brain development dorsal/ventral pattern formation regulation of gene expression spinal cord dorsal/ventral patterning spinal cord motor neuron differentiation telencephalon development pallium development subpallium development cerebral cortex radial glia guided migration neural tube development embryonic limb morphogenesis mammary gland development forebrain development tube development regulation of cell proliferation regulation of apoptotic process camera-type eye development regulation of cell differentiation negative regulation of cell differentiation embryonic morphogenesis anatomical structure formation involved in morphogenesis neuron fate commitment branching morphogenesis of an epithelial tube inner ear development anatomical structure development limb development
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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