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GLP-1 Antibody (710320) in WB
Western blot analysis of Glucagon in whole cell extracts from mouse small intestine using a Glucagon Recombinant Rabbit Superclonal™ Antibody (Product # 710320) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~21kDa.
Product Details
710320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
24HCLC
Immunogen
Peptide corresponding to amino acids 98-118 of human GLP-1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532687
Product Specific Information
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glicentin-related polypeptide; GLP1/2; Glucagon like peptide 1; glucagon-like peptide 1; glucagon-like peptide 2; MGC138331; OXY; preproglucagon; Pro-glucagon
Gene Aliases: GCG; GLP-1; GLP1; GLP2; Glu; GRPP; PPG
UniProt ID: (Human) P01275, (Mouse) P55095
Entrez Gene ID: (Human) 2641, (Mouse) 14526
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
feeding behavior
cell proliferation
protein kinase A signaling
positive regulation of peptidyl-threonine phosphorylation
positive regulation of cAMP biosynthetic process
positive regulation of protein binding
negative regulation of appetite
positive regulation of peptidyl-serine phosphorylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
response to starvation
negative regulation of apoptotic process
positive regulation of protein kinase activity
regulation of insulin secretion
positive regulation of histone H3-K4 methylation
positive regulation of ERK1 and ERK2 cascade
cellular response to glucagon stimulus
positive regulation of calcium ion import
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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