Proteintech

GLS Monoclonal Antibody (3A12A1)

GLS Antibody in Immunocytochemistry (ICC/IF)

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GLS Antibody (66265-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using KGA/GAC antibody (Product # 66265-1-Ig) (Clone: 3A12A1) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

GLS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GLS Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

66265-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3A12A1

Immunogen

GLS Fusion Protein Ag24227 (370-669 aa encoded by BC038507)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: YVGFSNATF QSERESGDRN FAIGYYLKEK KCFPEGTDMV GILDFYFQLC SIEVTCESAS VMAATLANGG FCPITGERVL SPEAVRNTLS LMHSCGMYDF SGQFAFHVGL PAKSGVAGGI LLVVPNVMGM MCWSPPLDKM GNSVKGIHFC HDLVSLCNFH NYDNLRHFAK KLDPRREGGD QRVKSVINLL FAAYTGDVSA LRRFALSAMD MEQRDYDSRT ALHVAAAEGH VEVVKFLLEA CKVNPFPKDR WNNTPMDEAL HFGHHDVFKI LQEYQVQYTP QGDSDNGKEN QTVHKNLDGL L (370-669 aa encoded by BC038507)

Target Information

Glutaminase is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing neurotransmitter glutamate, and maintaing acid-based balance in the kidney. There are alternate splicing results in multiple transcript variants in the Glutaminase gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prostate cancer cells demonstrate unique metabolism and substrate adaptability acutely after androgen deprivation therapy.

The Prostate

Filon MJ,Gillette AA,Yang B,Khemees TA,Skala MC,Jarrard DF

Thu Dec 01 00:00:00 UTC 2022

Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages.

Oxidative medicine and cellular longevity

Feng Y,Yang X,Huang J,Shen M,Wang L,Chen X,Yuan Y,Dong C,Ma X,Yuan F

Mon Apr 04 00:00:00 UTC 2022

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Glutamine uptake and catabolism is required for myofibroblast formation and persistence.

Journal of molecular and cellular cardiology

Gibb AA,Huynh AT,Gaspar RB,Ploesch TL,Lombardi AA,Lorkiewicz PK,Lazaropoulos MP,Bedi K,Arany Z,Margulies KB,Hill BG,Elrod JW

Tue Nov 01 00:00:00 UTC 2022

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

International journal of oncology

Terado T,Kim CJ,Ushio A,Minami K,Tambe Y,Kageyama S,Kawauchi A,Tsunoda T,Shirasawa S,Tanaka H,Inoue H

Thu Sep 01 00:00:00 UTC 2022

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Journal of hepatology

Dai W,Xu L,Yu X,Zhang G,Guo H,Liu H,Song G,Weng S,Dong L,Zhu J,Liu T,Guo C,Shen X

Fri May 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: DKFZp686O15119; FLJ10358; GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase

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Gene Aliases: 6330442B14; AAD20; AI314027; B230365M23Rik; GA; GAC; GAM; GLS; GLS1; Glut; KGA; KIAA0838; PAG; RATGLUT

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UniProt ID: (Human) O94925, (Mouse) D3Z7P3, (Rat) P13264

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Entrez Gene ID: (Pig) 399525, (Human) 2744, (Mouse) 14660, (Rat) 24398

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Function(s)

glutaminase activity protein binding hydrolase activity

Process(es)

suckling behavior regulation of respiratory gaseous exchange by neurological system process glutamate biosynthetic process glutamine catabolic process chemical synaptic transmission cellular amino acid biosynthetic process glutamate secretion protein homotetramerization glutamine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GLS Monoclonal Antibody (3A12A1)

GLS Antibody (66265-1-IG) in ICC/IF

Product Details

66265-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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