Proteintech

GLUD1 Polyclonal Antibody

GLUD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GLUD1 Antibody (14299-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue slide using (Product # 14299-1-AP) GLUD1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GLUD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14299-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Not applicable (N/A)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GLUD1 Fusion Protein Ag5694 (214-558 aa encoded by BC040132)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FIGPGIDVP APDMSTGERE MSWIADTYAS TIGHYDINAH ACVTGKPISQ GGIHGRISAT GRGVFHGIEN FINEASYMSI LGMTPGFGDK TFVVQGFGNV GLHSMRYLHR FGAKCIAVGE SDGSIWNPDG IDPKELEDFK LQHGSILGFP KAKPYEGSIL EADCDILIPA ASEKQLTKSN APRVKAKIIA EGANGPTTPE ADKIFLERNI MVIPDLYLNA GGVTVSYFEW LKNLNHVSYG RLTFKYERDS NYHLLMSVQE SLERKFGKHG GTIPIVPTAE FQDRISGASE KDIVHSGLAY TMERSARQIM RTAMKYNLGL DLRTAAYVNA IEKVFKVYNE AGVTFT (214-558 aa encoded by BC040132)

Target Information

Glutamate dehydrogenase, GluD1, is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis.

Nature metabolism

Park G,Haley JA,Le J,Jung SM,Fitzgibbons TP,Korobkina ED,Li H,Fluharty SM,Chen Q,Spinelli JB,Trivedi CM,Jang C,Guertin DA

Sat Jul 01 00:00:00 UTC 2023

Mandarin Fish (Siniperca chuatsi) p53 Regulates Glutaminolysis Induced by Virus via the p53/miR145-5p/c-Myc Pathway in Chinese Perch Brain Cells.

Microbiology spectrum

Ye C,Liu S,Li N,Zuo S,Niu Y,Lin Q,Liang H,Luo X,Fu X

Wed Apr 27 00:00:00 UTC 2022

Glutamine synthetase limits β-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1.

The Journal of clinical investigation

Dai W,Shen J,Yan J,Bott AJ,Maimouni S,Daguplo HQ,Wang Y,Khayati K,Guo JY,Zhang L,Wang Y,Valvezan A,Ding WX,Chen X,Su X,Gao S,Zong WX

Thu Dec 15 00:00:00 UTC 2022

NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression.

Frontiers in cell and developmental biology

Tsujimoto G,Ito R,Yoshikawa K,Ueki C,Okada N

Tue Sep 03 00:00:00 UTC 2024

Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival.

Blood cancer discovery

Li M,Teater MR,Hong JY,Park NR,Duy C,Shen H,Wang L,Chen Z,Cerchietti L,Davidson SM,Lin H,Melnick AM

Sat Jan 01 00:00:00 UTC 2022

The mTOR/PGC-1α/SIRT3 Pathway Drives Reductive Glutamine Metabolism to Reduce Oxidative Stress Caused by ISKNV in CPB Cells.

Microbiology spectrum

Fu X,Li K,Niu Y,Lin Q,Liang H,Luo X,Liu L,Li N

Wed Feb 23 00:00:00 UTC 2022

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Journal of immunology (Baltimore, Md. : 1950)

Fan TW,Daneshmandi S,Cassel TA,Uddin MB,Sledziona J,Thompson PT,Lin P,Higashi RM,Lane AN

Tue Nov 01 00:00:00 UTC 2022

Ginsenoside CK induces apoptosis in triple-negative breast cancer cells by targeting glutamine metabolism.

Biochemical pharmacology

Zhang B,Fu R,Duan Z,Shen S,Zhu C,Fan D

Mon Aug 01 00:00:00 UTC 2022

Ginsenoside CK induces apoptosis in triple-negative breast cancer cells by targeting glutamine metabolism.

Biochemical pharmacology

Zhang B,Fu R,Duan Z,Shen S,Zhu C,Fan D

Mon Aug 01 00:00:00 UTC 2022

Ginsenoside CK induces apoptosis in triple-negative breast cancer cells by targeting glutamine metabolism.

Biochemical pharmacology

Zhang B,Fu R,Duan Z,Shen S,Zhu C,Fan D

Mon Aug 01 00:00:00 UTC 2022

Ginsenoside CK induces apoptosis in triple-negative breast cancer cells by targeting glutamine metabolism.

Biochemical pharmacology

Zhang B,Fu R,Duan Z,Shen S,Zhu C,Fan D

Mon Aug 01 00:00:00 UTC 2022

Nicotinamide reprograms adipose cellular metabolism and increases mitochondrial biogenesis to ameliorate obesity.

The Journal of nutritional biochemistry

Luo C,Yang C,Wang X,Chen Y,Liu X,Deng H

Thu Sep 01 00:00:00 UTC 2022

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Pharmacological research

Wang Q,Wu M,Li H,Rao X,Ao L,Wang H,Yao L,Wang X,Hong X,Wang J,Aa J,Sun M,Wang G,Liu J,Zhou F

Tue Nov 01 00:00:00 UTC 2022

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Pharmacological research

Wang Q,Wu M,Li H,Rao X,Ao L,Wang H,Yao L,Wang X,Hong X,Wang J,Aa J,Sun M,Wang G,Liu J,Zhou F

Tue Nov 01 00:00:00 UTC 2022

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Pharmacological research

Wang Q,Wu M,Li H,Rao X,Ao L,Wang H,Yao L,Wang X,Hong X,Wang J,Aa J,Sun M,Wang G,Liu J,Zhou F

Tue Nov 01 00:00:00 UTC 2022

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Pharmacological research

Wang Q,Wu M,Li H,Rao X,Ao L,Wang H,Yao L,Wang X,Hong X,Wang J,Aa J,Sun M,Wang G,Liu J,Zhou F

Tue Nov 01 00:00:00 UTC 2022

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

International journal of oncology

Terado T,Kim CJ,Ushio A,Minami K,Tambe Y,Kageyama S,Kawauchi A,Tsunoda T,Shirasawa S,Tanaka H,Inoue H

Thu Sep 01 00:00:00 UTC 2022

Cytosolic GDH1 degradation restricts protein synthesis to sustain tumor cell survival following amino acid deprivation.

The EMBO journal

Shao J,Shi T,Yu H,Ding Y,Li L,Wang X,Wang X

Mon Oct 18 00:00:00 UTC 2021

Cytosolic GDH1 degradation restricts protein synthesis to sustain tumor cell survival following amino acid deprivation.

The EMBO journal

Shao J,Shi T,Yu H,Ding Y,Li L,Wang X,Wang X

Mon Oct 18 00:00:00 UTC 2021

Cytosolic GDH1 degradation restricts protein synthesis to sustain tumor cell survival following amino acid deprivation.

The EMBO journal

Shao J,Shi T,Yu H,Ding Y,Li L,Wang X,Wang X

Mon Oct 18 00:00:00 UTC 2021

PINCH-1 promotes Δ¹-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Amino acids

Cui C,Wang J,Guo L,Wu C

Wed Dec 01 00:00:00 UTC 2021

Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.

Frontiers in cell and developmental biology

Bai C,Wang H,Dong D,Li T,Yu Z,Guo J,Zhou W,Li D,Yan R,Wang L,Wang Z,Li Y,Ren L

Wed Apr 21 00:00:00 UTC 2021

Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury.

The Journal of nutrition

Blum JE,Gheller BJ,Benvie A,Field MS,Panizza E,Vacanti NM,Berry D,Thalacker-Mercer A

Tue Nov 02 00:00:00 UTC 2021

Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury.

The Journal of nutrition

Blum JE,Gheller BJ,Benvie A,Field MS,Panizza E,Vacanti NM,Berry D,Thalacker-Mercer A

Tue Nov 02 00:00:00 UTC 2021

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

International journal of molecular sciences

Beręsewicz-Haller M,Krupska O,Bochomulski P,Dudzik D,Chęcińska A,Hilgier W,Barbas C,Zablocki K,Zablocka B

Sat Aug 07 00:00:00 UTC 2021

Adaptation of pancreatic cancer cells to nutrient deprivation is reversible and requires glutamine synthetase stabilization by mTORC1.

Proceedings of the National Academy of Sciences of the United States of America

Tsai PY,Lee MS,Jadhav U,Naqvi I,Madha S,Adler A,Mistry M,Naumenko S,Lewis CA,Hitchcock DS,Roberts FR,DelNero P,Hank T,Honselmann KC,Morales Oyarvide V,Mino-Kenudson M,Clish CB,Shivdasani RA,Kalaany NY

Tue Mar 09 00:00:00 UTC 2021

The cross-talk between DDR1 and STAT3 promotes the development of hepatocellular carcinoma.

Aging

Lin Y,Jin H,Wu X,Jian Z,Zou X,Huang J,Guan R,Wei X

Mon Jul 27 00:00:00 UTC 2020

Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.

Cancer cell

Li M,Chiang YL,Lyssiotis CA,Teater MR,Hong JY,Shen H,Wang L,Hu J,Jing H,Chen Z,Jain N,Duy C,Mistry SJ,Cerchietti L,Cross JR,Cantley LC,Green MR,Lin H,Melnick AM

Mon Jun 10 00:00:00 UTC 2019

Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.

Molecular oncology

Hong R,Zhang W,Xia X,Zhang K,Wang Y,Wu M,Fan J,Li J,Xia W,Xu F,Chen J,Wang S,Zhan Q

Mon Apr 01 00:00:00 UTC 2019

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Cell reports

Bott AJ,Shen J,Tonelli C,Zhan L,Sivaram N,Jiang YP,Yu X,Bhatt V,Chiles E,Zhong H,Maimouni S,Dai W,Velasquez S,Pan JA,Muthalagu N,Morton J,Anthony TG,Feng H,Lamers WH,Murphy DJ,Guo JY,Jin J,Crawford HC,Zhang L,White E,Lin RZ,Su X,Tuveson DA,Zong WX

Tue Oct 29 00:00:00 UTC 2019

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 UTC 2018

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 UTC 2018

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

Journal of translational medicine

Han Q,Ma Y,Wang H,Dai Y,Chen C,Liu Y,Jing L,Sun X

Fri Jul 20 00:00:00 UTC 2018

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

Journal of translational medicine

Han Q,Ma Y,Wang H,Dai Y,Chen C,Liu Y,Jing L,Sun X

Fri Jul 20 00:00:00 UTC 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 UTC 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 UTC 2018

Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.

Science (New York, N.Y.)

Spinelli JB,Yoon H,Ringel AE,Jeanfavre S,Clish CB,Haigis MC

Fri Nov 17 00:00:00 UTC 2017

Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.

Science (New York, N.Y.)

Spinelli JB,Yoon H,Ringel AE,Jeanfavre S,Clish CB,Haigis MC

Fri Nov 17 00:00:00 UTC 2017

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Cell

Yang W,Nagasawa K,Münch C,Xu Y,Satterstrom K,Jeong S,Hayes SD,Jedrychowski MP,Vyas FS,Zaganjor E,Guarani V,Ringel AE,Gygi SP,Harper JW,Haigis MC

Thu Nov 03 00:00:00 UTC 2016

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Cell

Yang W,Nagasawa K,Münch C,Xu Y,Satterstrom K,Jeong S,Hayes SD,Jedrychowski MP,Vyas FS,Zaganjor E,Guarani V,Ringel AE,Gygi SP,Harper JW,Haigis MC

Thu Nov 03 00:00:00 UTC 2016

Differential Glutamate Metabolism in Proliferating and Quiescent Mammary Epithelial Cells.

Cell metabolism

Coloff JL,Murphy JP,Braun CR,Harris IS,Shelton LM,Kami K,Gygi SP,Selfors LM,Brugge JS

Tue May 10 00:00:00 UTC 2016

Differential Glutamate Metabolism in Proliferating and Quiescent Mammary Epithelial Cells.

Cell metabolism

Coloff JL,Murphy JP,Braun CR,Harris IS,Shelton LM,Kami K,Gygi SP,Selfors LM,Brugge JS

Tue May 10 00:00:00 UTC 2016

Blocking glutamate carboxypeptidase II inhibits glutamate excitotoxicity and regulates immune responses in experimental autoimmune encephalomyelitis.

The FEBS journal

Ha D,Bing SJ,Ahn G,Kim J,Cho J,Kim A,Herath KH,Yu HS,Jo SA,Cho IH,Jee Y

Thu Sep 01 00:00:00 UTC 2016

Blocking glutamate carboxypeptidase II inhibits glutamate excitotoxicity and regulates immune responses in experimental autoimmune encephalomyelitis.

The FEBS journal

Ha D,Bing SJ,Ahn G,Kim J,Cho J,Kim A,Herath KH,Yu HS,Jo SA,Cho IH,Jee Y

Thu Sep 01 00:00:00 UTC 2016

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 UTC 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 UTC 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 UTC 2016

Replication of the Shrimp Virus WSSV Depends on Glutamate-Driven Anaplerosis.

PloS one

Li CY,Wang YJ,Huang SW,Cheng CS,Wang HC

Tue Jul 19 00:00:00 UTC 2016

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 UTC 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 UTC 2015

Cerebral level of vGlut1 is increased and level of glycine is decreased in TgSwDI mice.

Journal of Alzheimer's disease : JAD

Timmer NM,Metaxas A,van der Stelt I,Kluijtmans LA,van Berckel BN,Verbeek MM

Wed Sep 03 00:00:00 UTC 2014

Cerebral level of vGlut1 is increased and level of glycine is decreased in TgSwDI mice.

Journal of Alzheimer's disease : JAD

Timmer NM,Metaxas A,van der Stelt I,Kluijtmans LA,van Berckel BN,Verbeek MM

Wed Sep 03 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: epididymis tissue sperm binding protein Li 18mP; GDH 1; glutamate dehydrogenase (NAD(P)+); Glutamate dehydrogenase 1, mitochondrial; Memory-related gene 2 protein; MRG-2

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Gene Aliases: Ac2-281; AI118167; GDH; Gdh-X; GDH1; GLUD; GLUD1; Gludeha; Gludl; MRG-2

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UniProt ID: (Human) P00367, (Mouse) P26443, (Rat) P10860

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Entrez Gene ID: (Human) 2746, (Mouse) 14661, (Rat) 24399

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Function(s)

glutamate dehydrogenase (NAD+) activity glutamate dehydrogenase [NAD(P)+] activity protein binding ATP binding GTP binding identical protein binding ADP binding NAD+ binding leucine binding nucleotide binding oxidoreductase activity enzyme binding

Process(es)

glutamate biosynthetic process glutamate catabolic process glutamine metabolic process cellular amino acid biosynthetic process substantia nigra development positive regulation of insulin secretion oxidation-reduction process tricarboxylic acid metabolic process cellular amino acid metabolic process response to aluminum ion long-term memory

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GLUD1 Polyclonal Antibody

GLUD1 Antibody (14299-1-AP) in IHC (P)

Product Details

14299-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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