Invitrogen

GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

FIGURE: 1 / 16

GLUT1 Antibody (MA5-31960)

Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GLUT1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960). GLUT-1 shows a streak like pattern in cell lines as seen in all the 3 lanes. The siRNA Lane shows almost 90% loss of s... View More

GLUT1 Antibody in Immunocytochemistry (ICC/IF)

GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GLUT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GLUT1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960). GLUT-1 shows a streak like pattern in cell lines as seen in all the 3 lanes. The siRNA Lane shows almost 90% loss of streak like signal. {KD}

Product Details

MA5-31960

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:5,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500

Flow Cytometry (Flow)

1:500-1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SA0377

Immunogen

Synthetic peptide within Human GLUT1 aa 443-492

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809254

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Glucose transporter 1 (GLUT1), encoded by the SLC2A1 gene, is a facilitative glucose transporter that plays a pivotal role in the uptake of glucose across cell membranes, particularly in tissues with high-energy demands such as the brain, erythrocytes, and endothelial cells of the blood-brain barrier. GLUT1 is ubiquitously expressed and functions through a passive diffusion mechanism, allowing glucose to move down its concentration gradient. Its involvement in maintaining glucose homeostasis is crucial for cellular metabolism and energy production. Mutations in SLC2A1 can lead to GLUT1 deficiency syndrome, characterized by seizures, developmental delay, and movement disorders due to impaired glucose transport into the brain. GLUT1 also plays a significant role in cancer biology, where its overexpression is commonly observed in various tumors, facilitating the enhanced glucose uptake required for rapid tumor cell proliferation. This characteristic makes GLUT1 a potential target for cancer diagnostics and therapeutics. Additionally, GLUT1's regulation is influenced by factors such as hypoxia, insulin, and cellular stress, highlighting its adaptive role in different physiological and pathological states.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.

Nature communications

Schmidt S,Stautner C,Vu DT,Heinz A,Regensburger M,Karayel O,Trümbach D,Artati A,Kaltenhäuser S,Nassef MZ,Hembach S,Steinert L,Winner B,Jürgen W,Jastroch M,Luecken MD,Theis FJ,Westmeyer GG,Adamski J,Mann M,Hiller K,Giesert F,Vogt Weisenhorn DM,Wurst W

MA5-31960 was used in Flow Cytometry to demonstrate a correlation between OGDHC activity in our cellular model and the disease progression.

Thu Nov 23 00:00:00 UTC 2023

Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesis.

Molecular oncology

Afsar SY,Alam S,Fernandez Gonzalez C,van Echten-Deckert G

MA5-31960 was used in Western Blotting to report on a newly identified role of the S1P/S1PR1-3 axis in glucose metabolism in SGPL1-deficient mouse embryonic fibroblasts.

Sat Oct 01 00:00:00 UTC 2022

Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesis.

Molecular oncology

Afsar SY,Alam S,Fernandez Gonzalez C,van Echten-Deckert G

MA5-31960 was used in Western Blotting to report on a newly identified role of the S1P/S1PR1-3 axis in glucose metabolism in SGPL1-deficient mouse embryonic fibroblasts.

Sat Oct 01 00:00:00 UTC 2022

Activation of Wnt/β-catenin pathway mitigates blood-brain barrier dysfunction in Alzheimer's disease.

Brain : a journal of neurology

Wang Q,Huang X,Su Y,Yin G,Wang S,Yu B,Li H,Qi J,Chen H,Zeng W,Zhang K,Verkhratsky A,Niu J,Yi C

MA5-31960 was used in Immunohistochemistry-immunofluorescence to show dysfunctional blood-brain barrier in patients with Alzheimer's disease reflected by perivascular accumulation of blood-derived fibrinogen in the hippocampus and cortex, accompanied by decreased tight junction proteins Claudin-5 and glucose transporter Glut-1 in the brain endothelial cells.

Mon Dec 19 00:00:00 UTC 2022

Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target.

Nature communications

Cho H,Kwon HY,Sharma A,Lee SH,Liu X,Miyamoto N,Kim JJ,Im SH,Kang NY,Chang YT

MA5-31960 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Immunohistochemistry (Frozen) to demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

Mon Oct 10 00:00:00 UTC 2022

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.

Scientific reports

Risha Y,Minic Z,Ghobadloo SM,Berezovski MV

MA5-31960 was used in Flow cytometry/Cell sorting to demonstrate that exosomes are a rich source of breast cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis.

Tue Aug 11 00:00:00 UTC 2020

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.

Scientific reports

Risha Y,Minic Z,Ghobadloo SM,Berezovski MV

Tue Aug 11 00:00:00 UTC 2020

Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.

Cell reports

Wang W,Kini A,Wang Y,Liu T,Chen Y,Vukmanic E,Emery D,Liu Y,Lu X,Jin L,Lee SJ,Scott P,Liu X,Dean K,Lu Q,Fortuny E,James R,Kaplan HJ,Du J,Dean DC

MA5-31960 was used in Immunohistochemistry-immunofluorescence to provide evidence that contact between externalized phosphatidylserine (PS) on OS tips and apical RPE receptors activates Akt, linking phagocytosis with glucose transport to photoreceptors for new OS synthesis.

Tue Jul 30 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

View more View less

Gene Aliases: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

View more View less

UniProt ID: (Human) P11166, (Mouse) P17809, (Rat) P11167

View more View less

Entrez Gene ID: (Human) 6513, (Mouse) 20525, (Rat) 24778

View more View less

Function(s)

glucose transmembrane transporter activity protein binding kinase binding dehydroascorbic acid transporter activity identical protein binding xenobiotic transporter activity protein self-association D-glucose transmembrane transporter activity transporter activity sugar:proton symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity

Process(es)

protein complex assembly transport carbohydrate transport glucose transport proton transport hexose transmembrane transport cellular response to glucose starvation xenobiotic transport glucose import transmembrane transport dehydroascorbic acid transport cation transmembrane transport lactose biosynthetic process response to osmotic stress L-ascorbic acid metabolic process regulation of insulin secretion glucose transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

GLUT1 Antibody (MA5-31960)

Product Details

MA5-31960

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!