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Invitrogen
GM-CSF Monoclonal Antibody (1089), Biotin
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GM-CSF Antibody (M501B) in ELISA
Sandwich ELISA analysis of human GM-CSF was performed using a Human GM-CSF Colorimetric ELISA kit (Product # EHGMCSF) by loading 50 µL per well of a mouse anti-human GM-CSF biotinylated antibody (Product # M501B) at a concentration of 0.05 µg/mL and 50 µL of human GM-CSF Recombinant Protein (Product # SGMCSF) at 600, 240, 96, 38.4, 15.4, and 0 pg/mL (both in quadruplicate) across a 9 µg/mL mouse anti-human GM-CSF (Product # M500A) pre-coated plate. The plate was incubated for 3 hours at room temperature, washed, and incubated with 100 µL p... View More
Product Details
M501B
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1089
Immunogen
Glycosylated, CHO-derived recombinant human GM-CSF
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with 4% BSA
Contains
proprietary Preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
RRID
AB_223572
Product Specific Information
M501B targets Human GM-CSF in ELISA applications and shows reactivity with Human samples.
The M501B immunogen is glycosylated, CHO-derived recombinant human GM-CSF.
M501B detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The M501B GMCSF antibody (biotinylated conjugate of clone 1089) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M500A (clone 3092). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody M501B (clone 1089) and antibody M500A (clone 3092) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications.
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; Molgramostin; Sargramostim
Gene Aliases: CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437
MAPK cascade
embryonic placenta development
immune response
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
peptidyl-tyrosine phosphorylation
positive regulation of interleukin-23 production
epithelial fluid transport
macrophage activation
positive regulation of tyrosine phosphorylation of Stat5 protein
myeloid dendritic cell differentiation
positive regulation of GTPase activity
positive regulation of DNA replication
negative regulation of cytolysis
cellular response to lipopolysaccharide
positive regulation of podosome assembly
dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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