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Invitrogen
GNAQ Monoclonal Antibody (13H4)
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GNAQ Antibody (MA5-49175) in IHC (P)
Immunohistochemistry analysis of GNAQ in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with GNAQ Monoclonal antibody (Product # MA5-49175) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-mouse IgG was us... View More
Product Details
MA5-49175
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
13H4
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GNAQ (102-138aa KYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWND).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074833
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Immunogen sequence is identical to the related mouse and rat sequences.
Positive Control - WB: human Jurkat whole cell, human Hela whole cell, human A549 whole cellhuman A431 whole cell, human MCF-7 whole cell, human K562 whole cell, monkey COS-7 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue. IHC: human ovarian cancer tissue, human ovarian cancer tissue, mouse testis tissue. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G alpha q; guanine nucleotide binding protein (G protein), q polypeptide; guanine nucleotide binding protein alpha q subunit; guanine nucleotide binding protein, alpha q polypeptide; guanine nucleotide regulatory protein G alpha q; Guanine nucleotide-binding protein alpha-q; Guanine nucleotide-binding protein G(q) subunit alpha; heterotrimeric guanine nucleotide-binding protein alpha q subunit
Gene Aliases: 1110005L02Rik; 6230401I02Rik; AA408290; AW060788; CMC1; Dsk1; Dsk10; G-ALPHA-q; Galphaq; GAQ; GNAQ; Gq; GqI; SWS
UniProt ID: (Human) P50148, (Rat) P82471, (Mouse) P21279
Entrez Gene ID: (Human) 2776, (Rat) 81666, (Mouse) 14682
skeletal system development
action potential
negative regulation of protein kinase activity
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of phospholipase C activity
G-protein coupled acetylcholine receptor signaling pathway
glutamate receptor signaling pathway
heart development
blood coagulation
phototransduction, visible light
entrainment of circadian clock
post-embryonic development
neuron remodeling
forebrain neuron development
platelet activation
regulation of catenin import into nucleus
maternal behavior
embryonic digit morphogenesis
positive regulation of GTPase activity
regulation of melanocyte differentiation
developmental pigmentation
protein stabilization
phospholipase C-activating dopamine receptor signaling pathway
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
metabolic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of potassium ion transport
positive regulation of smooth muscle cell proliferation
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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