Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 7
GNB1 Antibody (PA5-30046) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GNB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 Polyclonal Antibody (Product # PA5-30046) diluted at 1:500. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.
Product Details
PA5-30046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.44 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547520
Product Specific Information
Recommended positive controls: Jurkat, Raji, NCI-H929, Mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: beta subunit, signal-transducing proteins GS/GI; gbb1; guanine nucleotide binding protein (G protein), beta 1; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide binding protein, beta 1; Guanine nucleotide-binding protein beta 1; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein, beta-1 subunit; T beta; testicular tissue protein Li 72; Transducin beta chain 1
Gene Aliases: AA409223; C77571; Gnb-1; GNB1; MRD42
UniProt ID: (Human) P62873, (Mouse) P62874, (Rat) P54311
Entrez Gene ID: (Human) 2782, (Mouse) 14688, (Rat) 24400
signal transduction
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
phototransduction, visible light
cell proliferation
cardiac muscle cell apoptotic process
sensory perception of taste
retina development in camera-type eye
cellular response to hypoxia
protein folding
adenylate cyclase-activating dopamine receptor signaling pathway
G-protein coupled acetylcholine receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
Ras protein signal transduction
rhodopsin mediated signaling pathway
platelet activation
cellular response to glucagon stimulus
cellular response to prostaglandin E stimulus
cellular response to catecholamine stimulus
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support