Proteintech

GOLPH3 Polyclonal Antibody

GOLPH3 Antibody in Immunocytochemistry (ICC/IF)

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GOLPH3 Antibody (19112-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GOLPH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GOLPH3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19112-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GOLPH3 Fusion Protein Ag5443 (1-298 aa encoded by BC033725)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTSLTQRSS GLVQRRTEAS RNAADKERAA GGGAGSSEDD AQSRRDEQDD DDKGDSKETR LTLMEEVLLL GLKDREGYTS FWNDCISSGL RGCMLIELAL RGRLQLEACG MRRKSLLTRK VICKSDAPTG DVLLDEALKH VKETQPPETV QNWIELLSGE TWNPLKLHYQ LRNVRERLAK NLVEKGVLTT EKQNFLLFDM TTHPLTNNNI KQRLIKKVQE AVLDKWVNDP HRMDRRLLAL IYLAHASDVL ENAFAPLLDE QYDLATKRVR QLLDLDPEVE CLKANTNEVL WAVVAAFTK (1-298 aa encoded by BC033725 )

Target Information

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long Intergenic Noncoding RNA 00641 Promotes Growth and Invasion of Colorectal Cancer through Regulating miR-450b-5p/GOLPH3 Axis.

Journal of oncology

Hong Z,Pan J,Chen M,Deng X,Chen Z,Wang C,Qiu C

Sat Jul 16 00:00:00 UTC 2022

Golgi phosphoprotein 3 induces autophagy and epithelial-mesenchymal transition to promote metastasis in colon cancer.

Cell death discovery

Gong LY,Tu T,Zhu J,Hu AP,Song JW,Huang JQ,Yang Y,Zhu Z,Chen Y

Mon Feb 21 00:00:00 UTC 2022

Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma.

Bioengineered

Zou C,Li X,Wei H,Wu S,Song J,Tang Z,Luo H,Lv X,Ai Y

Fri Apr 01 00:00:00 UTC 2022

Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma.

Bioengineered

Zou C,Li X,Wei H,Wu S,Song J,Tang Z,Luo H,Lv X,Ai Y

Fri Apr 01 00:00:00 UTC 2022

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Communications biology

Yue X,Tiwari N,Zhu L,Ngo HDT,Lim JM,Gim B,Jing S,Wang Y,Qian Y,Lee I

Tue Dec 07 00:00:00 UTC 2021

GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer.

Cell death & disease

Song JW,Zhu J,Wu XX,Tu T,Huang JQ,Chen GZ,Liang LY,Zhou CH,Xu X,Gong LY

Thu Oct 21 00:00:00 UTC 2021

Golgi phosphoprotein 3 promotes the proliferation of gallbladder carcinoma cells via regulation of the NLRP3 inflammasome.

Oncology reports

Zhu Z,Zhu Q,Cai D,Chen L,Xie W,Bai Y,Luo K

Tue Jun 01 00:00:00 UTC 2021

A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma.

Cancer research

Wu S,Zhang L,Deng J,Guo B,Li F,Wang Y,Wu R,Zhang S,Lu J,Zhou Y

Wed Jul 01 00:00:00 UTC 2020

APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.

Cell reports

Uzureau S,Lecordier L,Uzureau P,Hennig D,Graversen JH,Homblé F,Mfutu PE,Oliveira Arcolino F,Ramos AR,La Rovere RM,Luyten T,Vermeersch M,Tebabi P,Dieu M,Cuypers B,Deborggraeve S,Rabant M,Legendre C,Moestrup SK,Levtchenko E,Bultynck G,Erneux C,Pérez-Morga D,Pays E

Tue Mar 17 00:00:00 UTC 2020

FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.

Frontiers in oncology

Yu LN,Liu Z,Tian Y,Zhao PP,Hua X

Wed Sep 30 00:00:00 UTC 2020

STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Wu F,Gao P,Wu W,Wang Z,Yang J,Di J,Jiang B,Su X

Wed Jul 11 00:00:00 UTC 2018

STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Wu F,Gao P,Wu W,Wang Z,Yang J,Di J,Jiang B,Su X

Wed Jul 11 00:00:00 UTC 2018

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 UTC 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 UTC 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 UTC 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 UTC 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 UTC 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 UTC 2020

Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology.

Cytoskeleton (Hoboken, N.J.)

Bruun K,Beach JR,Heissler SM,Remmert K,Sellers JR,Hammer JA

Mon May 01 00:00:00 UTC 2017

Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology.

Cytoskeleton (Hoboken, N.J.)

Bruun K,Beach JR,Heissler SM,Remmert K,Sellers JR,Hammer JA

Mon May 01 00:00:00 UTC 2017

GOLPH3 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in epithelial ovarian cancer.

Cancer medicine

Sun J,Yang X,Zhang R,Liu S,Gan X,Xi X,Zhang Z,Feng Y,Sun Y

Sat Apr 01 00:00:00 UTC 2017

GOLPH3 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in epithelial ovarian cancer.

Cancer medicine

Sun J,Yang X,Zhang R,Liu S,Gan X,Xi X,Zhang Z,Feng Y,Sun Y

Sat Apr 01 00:00:00 UTC 2017

GOLPH3 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in epithelial ovarian cancer.

Cancer medicine

Sun J,Yang X,Zhang R,Liu S,Gan X,Xi X,Zhang Z,Feng Y,Sun Y

Sat Apr 01 00:00:00 UTC 2017

GOLPH3 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in epithelial ovarian cancer.

Cancer medicine

Sun J,Yang X,Zhang R,Liu S,Gan X,Xi X,Zhang Z,Feng Y,Sun Y

Sat Apr 01 00:00:00 UTC 2017

miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.

Theranostics