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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GOT2 Antibodies

          Invitrogen

          GOT2 Monoclonal Antibody (3E9)

          Advanced Verification
          1 Published Figure
          2 References
          View all (23) GOT2 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite GOT2 Monoclonal Antibody (3E9)

          • Antibody Testing Data (4)
          • Published Figures (1)
          • Advanced Verification (1)
          GOT2 Antibody in Immunocytochemistry (ICC/IF)
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          GOT2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          GOT2 Antibody (MA5-15595) in ICC/IF

          Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using GOT2 monoclonal antibody (Product # MA5-15595) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          GOT2 Antibody in Immunocytochemistry (ICC/IF)
          GOT2 Antibody in Immunocytochemistry (ICC/IF)
          GOT2 Antibody in Western Blot (WB)
          GOT2 Antibody in Western Blot (WB)
          GOT2 Antibody in Western Blot (WB)
          GOT2 Antibody

          Product Details

          MA5-15595

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:200-1:1,000
          -

          ELISA (ELISA)

          1:10,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3E9

          Immunogen

          Purified recombinant fragment of human GOT2 expressed in E. Coli.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.03% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10981108

          Product Specific Information

          MA5-15595 targets GOT2 in indirect ELISA, IF and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

          The MA5-15595 immunogen is purified recombinant fragment of human GOT2 expressed in E. Coli.

          MA5-15595 detects GOT2 which has a predicted molecular weight of approximately 47kDa.

          Target Information

          GOT2 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; aspartate transaminase 2; FABP-1; FABPpm; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase 2, mitochondrial (aspartate aminotransferase 2); glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; mAspAT; mitochondrial aspartate aminotransferase; plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A

          View more View less

          Gene Aliases: AL022787; ASPATA; FABP-pm; Got-2; GOT2; KAT4; KATIV; KYAT4; Maat; mAspAT; mitAAT

          View more View less

          UniProt ID: (Human) P00505, (Mouse) P05202, (Rat) P00507

          View more View less

          Entrez Gene ID: (Human) 2806, (Mouse) 14719, (Rat) 25721

          View more View less

          Function(s)
          L-aspartate:2-oxoglutarate aminotransferase activity phospholipid binding kynurenine-oxoglutarate transaminase activity amino acid binding enzyme binding pyridoxal phosphate binding identical protein binding protein homodimerization activity poly(A) RNA binding L-phenylalanine:2-oxoglutarate aminotransferase activity catalytic activity transaminase activity transferase activity carboxylic acid binding
          Process(es)
          oxaloacetate metabolic process cellular amino acid metabolic process aspartate metabolic process aspartate biosynthetic process aspartate catabolic process transport lipid transport biosynthetic process glutamate catabolic process to aspartate glutamate catabolic process to 2-oxoglutarate dicarboxylic acid metabolic process response to ethanol gluconeogenesis 2-oxoglutarate metabolic process glutamate metabolic process cellular amino acid biosynthetic process fatty acid transport 4-hydroxyproline catabolic process L-kynurenine metabolic process
          It has to be done as per old AB suggested Products section.
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