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GPAA1 Antibody (PA5-89485) in WB
Western blot analysis of GPAA1 in extracts of Mouse heart. Samples were incubated with GPAA1 Polyclonal antibody (Product # PA5-89485) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-89485
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 90-310 of human GPAA1 (NP_0037921)
Conjugate
Form
Liquid
Concentration
0.88 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805601
Product Specific Information
Immunogen sequence: AWLERTMRSV GLEVYTQSFS RKLPFPDETH ERYMVSGTNV YGILRAPRAA STESLVLTVP CGSDSTNSQA VGLLLALAAH FRGQIYWAKD IVFLVTEHDL LGTEAWLEAY HDVNVTGMQS SPLQGRAGAI QAAVALELSS DVVTSLDVAV EGLNGQLPNL DLLNLFQTFC QKGGLLCTLQ GKLQPEDWTS LDGPLQGLQT LLLMVLRQAS GRPHGSHGLF L; Positive Samples: Mouse heart; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
Target Information
Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GAA1 protein homolog; Glycosylphosphatidylinositol anchor attachment 1 protein; GPI anchor attachment protein 1; mGAA1
Gene Aliases: C80044; Gaa1; Gpaa1; mGAA1
UniProt ID: (Mouse) Q9WTK3
Entrez Gene ID: (Mouse) 14731
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