Invitrogen

GPAM Polyclonal Antibody

GPAM Antibody in Immunohistochemistry (IHC)

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GPAM Antibody (PA5-20524) in IHC

Immunofluorescence of GPAT1 in mouse brain tissue with GPAM Polyclonal Antibody (Product # PA5-20524) at 20 µg/mL. Green: GPAT1 Blue: DAPI staining

Product Image Gallery

GPAM Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using GPAM antibody (Product # PA5-20524), shows increased expression of proteins in MSC upon differentiation to adipocytes. {TM}

Product Details

PA5-20524

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

2.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid peptide near the carboxy terminus of the human GPAT1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11155813

Product Specific Information

A suggested positive control is rat brain tissue lysate.

PA5-20524 can be used with blocking peptide PEP-0644.

Target Information

Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

One week of overeating upregulates angiogenic and lipolytic gene expression in human subcutaneous adipose tissue from exercise trained and untrained adults.

Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme

Ludzki AC,Krueger EM,Gillen JB,Taylor NM,Middlebrook DO,Baldwin TC,Karabetsos KC,Schleh MW,Horowitz JF

PA5-20524 was used in Western Blotting to indicate that a brief and modest overeating stimulus can impair insulin sensitivity and upregulate genes involved in abdominal adipose tissue storage capacity similarly in exercisers and nonexercisers.

Sat Oct 01 00:00:00 UTC 2022

Exercise training remodels subcutaneous adipose tissue in adults with obesity even without weight loss.

The Journal of physiology

Ahn C,Ryan BJ,Schleh MW,Varshney P,Ludzki AC,Gillen JB,Van Pelt DW,Pitchford LM,Howton SM,Rode T,Hummel SL,Burant CF,Little JP,Horowitz JF

PA5-20524 was used in Western Blotting to report that both 12 weeks of moderate-intensity continuous training (MICT) and 12 weeks of high-intensity interval training (HIIT) induced modifications in adipose tissue structure and factors that regulate adipose tissue remodelling, metabolism and the inflammatory signal pathway in adults with obesity, even without weight loss (with no meaningful differences between MICT and HIIT).

Sun May 01 00:00:00 UTC 2022

Moderate-Intensity Exercise and High-Intensity Interval Training Affect Insulin Sensitivity Similarly in Obese Adults.

The Journal of clinical endocrinology and metabolism

Ryan BJ,Schleh MW,Ahn C,Ludzki AC,Gillen JB,Varshney P,Van Pelt DW,Pitchford LM,Chenevert TL,Gioscia-Ryan RA,Howton SM,Rode T,Hummel SL,Burant CF,Little JP,Horowitz JF

PA5-20524 was used in Western Blot to compare the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on insulin sensitivity and other important metabolic adaptations in adults with obesity.

Sat Aug 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: glycerol 3-phosphate acyltransferase, mitochondrial; Glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT; GPAT-1; P90; RP11-426E5.2

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Gene Aliases: GPAM; GPAT; GPAT1; KIAA1560

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UniProt ID: (Human) Q9HCL2, (Mouse) Q61586, (Rat) P97564

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Entrez Gene ID: (Human) 57678, (Mouse) 14732, (Rat) 29653

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Function(s)

glycerol-3-phosphate O-acyltransferase activity O-acyltransferase activity transferase activity transferase activity, transferring acyl groups

Process(es)

lipid metabolic process fatty acid metabolic process acyl-CoA metabolic process triglyceride metabolic process glycerophospholipid metabolic process metabolic process phospholipid biosynthetic process response to glucose positive regulation of triglyceride biosynthetic process response to activity triglyceride biosynthetic process cellular response to insulin stimulus positive regulation of multicellular organism growth positive regulation of activated T cell proliferation cellular lipid metabolic process regulation of cytokine secretion defense response to virus fatty acid homeostasis phospholipid homeostasis negative regulation of activation-induced cell death of T cells interleukin-2 secretion response to fructose CDP-diacylglycerol biosynthetic process response to nutrient levels response to cadmium ion phosphatidic acid biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GPAM Polyclonal Antibody

GPAM Antibody (PA5-20524) in IHC

Product Details

PA5-20524

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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