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Invitrogen
GPNMB Polyclonal Antibody
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GPNMB Antibody (PA5-47301) in Neu
Neutralization of GPNMB in SVEC4‚10 mouse vascular endothelial cell line. Samples were incubated in GPNMB polyclonal antibody (Product # PA5-47301). Recombinant Mouse Osteoactivin/GPNMB Fc Chimera, immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc, supports the adhesion of the SVEC4‚10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL)... View More
Product Details
PA5-47301
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB Lys23-Asn502
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605801
Product Specific Information
In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; iris pigment dispersion; Osteoactivin; Transmembrane glycoprotein NMB
Gene Aliases: DC-HIL; Dchil; Gpnmb; Hgfin; ipd; Nmb
UniProt ID: (Mouse) Q99P91
Entrez Gene ID: (Mouse) 93695
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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