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Invitrogen
GPNMB Polyclonal Antibody
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GPNMB Antibody (PA5-89716) in WB
Western blot analysis of GPNMB in SK-MEL-28 cells. Samples were incubated with GPNMB Polyclonal antibody (Product # PA5-89716) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 23-486 of human GPNMB (NP_0010053401)
Conjugate
Form
Liquid
Concentration
2.25 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805741
Product Specific Information
Positive Samples: A-549, K-562, U-87MG, Mouse brain, Mouse liver, Rat brain, Rat liver; Cellular Location: Cell membrane, Melanosome, Single-pass type I membrane protein
Immunogen sequence: KRFHDVLGNE RPSAYMREHN QLNGWSSDEN DWNEKLYPVW KRGDMRWKNS WKGGRVQAVL TSDSPALVGS NITFAVNLIF PRCQKEDANG NIVYEKNCRN EAGLSADPYV YNWTAWSEDS DGENGTGQSH HNVFPDGKPF PHHPGWRRWN FIYVFHTLGQ YFQKLGRCSV RVSVNTANVT LGPQLMEVTV YRRHGRAYVP IAQVKDVYVV TDQIPVFVTM FQKNDRNSSD ETFLKDLPIM FDVLIHDPSH FLNYSTINYK WSFGDNTGLF VSTNHTVNHT YVLNGTFSLN LTVKAAAPGP CPPPPPPPRP SKPTPSLATT LKSYDSNTPG PAGDNPLELS RIPDENCQIN RYGHFQATIT IVEGILEVNI IQMTDVLMPV PWPESSLIDF VVTCQGSIPT EVCTIISDPT CEITQNTVCS PVDVDEMCLL TVRRTFNGSG TYCVNLTLGD DTSLALTSTL ISVP
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; iris pigment dispersion; Osteoactivin; transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB
Gene Aliases: DC-HIL; Dchil; GPNMB; HGFIN; ipd; NMB; UNQ1725/PRO9925
UniProt ID: (Human) Q14956, (Mouse) Q99P91
Entrez Gene ID: (Human) 10457, (Rat) 113955, (Mouse) 93695
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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