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GPR150 Antibody (PA5-102063) in ICC/IF
Immunocytochemistry analysis of GPR150 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GPR150 polyclonal antibody (Product # PA5-102063) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102063
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GPR150(Accession Q8NGU9), corresponding to amino acid residues L49-G99.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851493
Product Specific Information
Antibody detects endogenous levels of total GPR150.
Target Information
G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 150 (GPR150), also designated PGR11 and seven transmembrane helix receptor, is a member of the rhodopsin family of GCPRs and is involved in signal transduction. GPR150 is expressed in various human tissues, including normal small intestine, skeletal muscle, kidney and tonsil, as well as cancerous blood, bladder, placenta and parathyroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GPRv40; Probable G-protein coupled receptor 150; seven transmembrane helix receptor
Gene Aliases: GPR150; PGR11
UniProt ID: (Human) Q8NGU9
Entrez Gene ID: (Human) 285601
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