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Invitrogen
GPR177 Polyclonal Antibody
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GPR177 Antibody (PA5-110582) in WB
Western blot analysis of GPR177 in extracts of various cell lines. Samples were incubated with GPR177 Polyclonal antibody (Product # PA5-110582) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-110582
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 43-232 of human WLS (NP_0010022923)
Conjugate
Form
Liquid
Concentration
1.17 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855993
Product Specific Information
Immunogen sequence: YMSVKCVDAR KNHHKTKWFV PWGPNHCDKI RDIEEAIPRE IEANDIVFSV HIPLPHMEMS PWFQFMLFIL QLDIAFKLNN QIRENAEVSM DVSLAYRDDA FAEWTEMAHE RVPRKLKCTF TSPKTPEHEG RYYECDVLPF MEIGSVAHKF YLLNIRLPVN EKKKINVGIG EIKDIRLVGI HQNGGFTKVW
Target Information
Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: evenness interrupted homolog; EVI; G protein-coupled receptor 177; Integral membrane protein GPR177; Protein evenness interrupted homolog; Protein wntless homolog; Putative NF-kappa-B-activating protein 373; putative NFkB activating protein 373
Gene Aliases: 5031439A09Rik; AI173978; AI987742; C1orf139; EVI; GPR177; mig-14; MRP; UNQ85/PRO18667; WLS
UniProt ID: (Human) Q5T9L3, (Mouse) Q6DID7, (Rat) Q6P689
Entrez Gene ID: (Human) 79971, (Mouse) 68151, (Rat) 362065
mesoderm formation
multicellular organism development
anterior/posterior axis specification
Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
biological_process
intracellular protein transport
positive regulation of Wnt signaling pathway
midbrain development
hindbrain development
exocrine pancreas development
positive regulation of I-kappaB kinase/NF-kappaB signaling
Wnt protein secretion
positive regulation of Wnt protein secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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