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GPR18 (extracellular) Antibody (AGR-068-200UL) in IHC (F)
Expression of GPR18 in rat dorsomedial hypothalamus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR18 (extracellular) Antibody (#AGR-068), (1:1000), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR18 immunoreactivity (green) appears in neurons (vertical arrows) and along the wall of the 3rd ventricle (horizontal arrow). B. Pre-incubation of the Antibody with GPR18... View More
Product Details
AGR-068-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)HNQLDLSNGSHPEEYK, corresponding to amino acid residues 7-22 of mouse GPR18, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. GPR18 is an orphan G protein-coupled receptor found at high levels in the testis, small intestines and cells associated with the immune system including lymphocytes, thymus and spleen. GPR18 has been found to be a receptor for endogenous lipid neurotransmitters, several of which also bind to cannabinoid receptors. Some ligands described to be GPR18 agonists or partial agonists include N-Arachidonoylglycine, Abnormal cannabidiol, O-1602, AM-251, Cannabidiol, delta 9-Tetrahydrocannabinol, N-arachidonoyl ethanolamine and Arachidonylcyclopropylamide. Antagonists include the synthetic cannabidiol related compounds CID-85469571 and O-1918.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein coupled receptor 18; gpr18; gpr18 {ECO:0000312|EMBL:AAI28783.1}; N-arachidonyl glycine receptor; NAGly receptor; RP11-461N23.8
Gene Aliases: Gpr18
UniProt ID: (Rat) A1A5S3, (Mouse) Q8K1Z6
Entrez Gene ID: (Rat) 679957, (Mouse) 110168
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