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Invitrogen
GPR30 Recombinant Rabbit Monoclonal Antibody (20H15L21)
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FIGURE: 1 / 5
GPR30 Antibody (703480) in ICC/IF
HEK-293 cells transiently transfected with GPR30 (GPER1) were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-GPR30 (GPER1) Recombinant Rabbit Monoclonal Antibody (Product # 703480, 1:500 dilution). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h ... View More
Product Details
703480
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
20H15L21
Immunogen
Peptides corresponding to human GPR30 [1) aa353-aa375 2) aa2-aa19]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2866461
Product Specific Information
This antibody is predicted to react with Mouse, Rat.
Target Information
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chemoattractant receptor-like 2; chemokine receptor-like 2; constitutively expressed peptide-like receptor; FEG-1; Flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; G-protein coupled estrogen receptor 1; G-protein coupled receptor 30; GPCR-Br; heptahelix receptor; IL8-related receptor DRY12; leucine rich protein in GPR30 3'UTR; LYGPR; Lymphocyte-derived G-protein coupled receptor; Membrane estrogen receptor; mER
Gene Aliases: CEPR; CMKRL2; DRY12; FEG-1; GPCR-Br; GPER; GPER1; GPR30; LERGU; LERGU2; LyGPR; mER
UniProt ID: (Human) Q99527
Entrez Gene ID: (Human) 2852
positive regulation of protein phosphorylation
positive regulation of neurotransmitter secretion
negative regulation of leukocyte activation
inflammatory response
cell cycle
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
positive regulation of gene expression
negative regulation of gene expression
negative regulation of cell cycle process
positive regulation of phosphatidylinositol 3-kinase signaling
neuronal action potential
apoptotic chromosome condensation
nuclear fragmentation involved in apoptotic nuclear change
positive regulation of cell migration
intracellular steroid hormone receptor signaling pathway
positive regulation of cAMP biosynthetic process
mineralocorticoid receptor signaling pathway
positive regulation of insulin secretion
positive regulation of inositol trisphosphate biosynthetic process
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
steroid hormone mediated signaling pathway
positive regulation of MAPK cascade
innate immune response
negative regulation of fat cell differentiation
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of neurogenesis
negative regulation of DNA metabolic process
negative regulation of lipid biosynthetic process
positive regulation of release of sequestered calcium ion into cytosol
regulation of cytosolic calcium ion concentration
negative regulation of protein kinase B signaling
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of uterine smooth muscle contraction
negative regulation of cell cycle arrest
cellular response to glucose stimulus
cellular response to tumor necrosis factor
cellular response to peptide hormone stimulus
cellular response to mineralocorticoid stimulus
cellular response to estradiol stimulus
positive regulation of establishment of protein localization to plasma membrane
positive regulation of release of cytochrome c from mitochondria
negative regulation of vascular smooth muscle cell proliferation
positive regulation of endothelial cell apoptotic process
positive regulation of cardiac vascular smooth muscle cell differentiation
positive regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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