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GPR4 (extracellular) Antibody (AGR-041-200UL) in IHC (F)
Expression ofGPR4inrat dorsal raphe nucleus (DRN). Immunohistochemicalstaining of perfusion-fixed frozenratbrainsections with Anti-GPR4 (extracellular) Antibody &(8)239; (#AGR-041), (1:400), followed bygoat Anti-rabbit-Alexa-488.A.GPR4immunoreactivity (green)appears incells of both medial and lateral DRN (arrows).B. Pre-incubation of the Antibody with GPR4 (extracellular) Blocking Peptide (#BLP-GR041),suppr... View More
Product Details
AGR-041-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4, 2nd extracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR4 is a member of this family (subfamily lysophospholipid/lysosphingolipid) and its expression has been reported in kidney, heart, and especially lung, where it is at least five-fold greater than in other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein coupled receptor 19; G-protein coupled receptor 4; G-protein coupled receptor 6C.l; Gpr6c; GPR6C.l
Gene Aliases: GPR4
UniProt ID: (Human) P46093, (Mouse) Q8BUD0, (Rat) Q4KLH9
Entrez Gene ID: (Human) 2828, (Mouse) 319197, (Rat) 308408
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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