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GPR4 (extracellular) Antibody (AGR-041-F-50UL) in Flow
Cell surface detection of GPR4 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). (green) Cells + Anti-GPR4 (extracellular)-FITC Antibody (#AGR-041-F), (5 µg).
Product Details
AGR-041-F-50UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4, Extracellular, 2nd loop
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 15 µL or 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
Target Information
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR4 is a member of this family (subfamily lysophospholipid/lysosphingolipid) and its expression has been reported in kidney, heart, and especially lung, where it is at least five-fold greater than in other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein coupled receptor 19; G-protein coupled receptor 4; G-protein coupled receptor 6C.l; Gpr6c; GPR6C.l
Gene Aliases: GPR4
UniProt ID: (Human) P46093, (Mouse) Q8BUD0, (Rat) Q4KLH9
Entrez Gene ID: (Human) 2828, (Mouse) 319197, (Rat) 308408
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